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Generation of a Sprague-Dawley-GFP rat iPS cell line.

Stem cell research (2017-07-06)
Giulia Coppiello, Gloria Abizanda, Natalia Aguado, Elena Iglesias, Olalla Iglesias-Garcia, Antonio Lo Nigro, Felipe Prosper, Xabier L Aranguren
ZUSAMMENFASSUNG

We generated a rat iPSC line called ATCi-rSD95 from transgenic Sprague-Dawley GFP fetal fibroblasts. Established ATCi-rSD95 cells present a normal karyotype, silencing of the transgenes and express pluripotency-associated markers. Additionally, ATCi-rSD95 cells are able to form teratoma with differentiated cells derived from the three germ-layers that maintain the GFP expression.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Anti-NeuN-Antikörper, Klon A60, clone A60, Chemicon®, from mouse
Sigma-Aldrich
Alkaline Phosphatase Blue Membrane Substrate Solution, sufficient for 400 mL working substrate