52977-U
HybridSPE® DPX Tip
Hamilton, Tip volume 300 μL, box of 96 tips, bed weight 30 mg
Synonym(e):
HybridSPE® DPX SPE Tips
About This Item
Empfohlene Produkte
product name
HybridSPE® DPX Tip, weight 30 mg (bed), Hamilton, tip volume 300 μL
Produktlinie
HybridSPE®
Zusammensetzung
HybridSPE® sorbent
Verpackung
box of 96 tips
Methode(n)
solid phase extraction (SPE): suitable
Spitzenvolumen
300 μL
Gewicht
30 mg (bed)
Aktive Matrixgruppe
Zirconia-based phase
Anwendung(en)
food and beverages
Kompatibilität
Hamilton
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Allgemeine Beschreibung
In this simple technique, biological plasma or serum is first subjected to protein precipitation via the addition and mixing of acidified acetonitrile. Precipitated proteins are then removed by centrifugation and the resulting supernatant is extracted using the HybridSPE DPX tip which acts as a chemical filter that specifically targets the removal of endogenous sample phospholipids.
The phospholipid retention mechanism is based on a highly selective Lewis acid-base interaction between the proprietary zirconia ions functionally bonded to the HybridSPE® stationary phase and the phosphate moiety consistent with all phospholipids. The resulting eluent is ready for immediate LC/MS or LC/MS/MS analysis.
We offer an array of tips that are compatible with the most common liquid handling platforms and pipettors on the market: Tecan, Hamilton, INTEGRA, and a Universal format as well.
What size tips do I need?
There are two versions of the DPX tips available for the HybridSPE-PL. The standard HybridSPE DPX 50 mg tips are designed for use with biological sample volumes between 100-300 μL, while the HybridSPE DPX 30 mg tips are designed for use with biological sample volumes between 30-100 μL.
Guideline for 30 mg tips - plasma/serum sample: 30-100 μL; precipitating agent: 90-300 μL
Guideline for 50 mg tips - plasma/serum sample: 100-300 μL; precipitating agent: 300-900 μL
Leistungsmerkmale und Vorteile
- Minimal elution solvent volumes
- Rapid extraction times (less than 3 minutes/sample)
- High extraction efficiencies
- Easy to perform extractions
- Lower costs
- Higher throughput
- Minimal training required
- Environmentally friendly
- Eliminates ion suppression effects often associated with phospholipids
- Tips offered to fit many formats: Tecan, Hamilton®, INTEGRA, and Universal liquid handling platforms and pipettors.
Rechtliche Hinweise
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Verwandter Inhalt
Die Festphasenextraktion (SPE) ist eine Technik zur schnellen, selektiven Probenvorbereitung und -aufreinigung vor einer chromatographischen Analyse (z. B. HPLC, GC, DC). Mit der SPE können Probenmatrizes ausgetauscht, Analyten konzentriert und Interferenzen und Verunreinigungen entfernt werden, um Ergebnisse zu verbessern und die analytische Säule zu schützen.
Solid phase extraction purifies samples for chromatographic analysis, improving data quality and protecting analytical columns.
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