SHP002

CRISPR & MISSION^® Lentiviral Packaging Mix

• Synonym(e): Lentiviral Packaging Mix
• UNSPSC-Code: 41106609
• NACRES: NA.51

Eigenschaften

• Konzentration: 100 ng/μL (in Tris-EDTA)
• Qualitätsniveau: 200
• Anwendung(en): CRISPR
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

The CRISPR & MISSION Lentiviral Packaging Mix is an optimized formulation of two plasmids expressing the key HIV packaging genes and a heterologous viral envelope gene.

Lentiviral particles are generated from three main components:

• The packaging vector, which contains the minimal set of lentiviral genes required to generate the virion structural proteins and packaging functions.
• The vesicular stomatitis virus G-protein envelope vector, which provides the heterologous envelope for pseudotyping and allows these lentiviral particles to efficiently deliver the transfer sequence of interest to a wide variety of cell types, including primary and non-dividing cells
• The lentiviral transfer vector, which contains the sequence of interest as well as the cis acting sequences necessary for packaging.

The Lentiviral Packaging Mix contains the first two components; it is designed to be co-transfected along with a compatible lentiviral transfer vector in order to create high-titer pseudo-typed lentiviral particles used for downstream transduction applications. This product is recommended for packaging Lenti CRISPR, Lenti ORF, and Lenti shRNA vectors.

Anwendung

Functional Genomics/Screening/Target Validation

Rechtliche Hinweise

CRISPR Use License Agreement

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable