SEP020

Seppro^® IgY14

LC2

• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Säule D × L: 6.4 mm × 63.0 mm
• Kapazität: 2.5 mg total protein loading(35 μL of human plasma, average human protein concentration 70 mg/mL)
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

The targeted highly abundant proteins are simultaneously removed by the immobilized specific IgYs when crude biological samples are passed through the column. Selective immunodepletion provides an enriched pool of low abundance proteins for downstream proteomic analyses. Specific removal of these 14 highly abundant proteins depletes ~95% of the total protein mass from human serum or plasma. The low abundance proteins in the flow-through fractions can then be studied. Removal of highly abundant proteins enables improved resolution and dynamic range for one dimensional electrophoresis (1DGE), two-dimensional (2DGE) electrophoresis and liquid chromatography/ mass spectrometry (LC/MS). The collected flow-through fractions may need to be concentrated dependent upon the downstream application.

Seppro^® IgY14-Säulen ermöglichen die Trennung von 14 hochabundanten Proteinen (HAP) aus Plasmaprotein-Mischungen. Die aviären polyklonalen IgY (Immunglobulin aus Eigelb)-Antikörper bieten aufgrund ihrer hochspezifischen Partitionierungseigenschaften wesentliche Vorteile gegenüber Konkurrenzprodukten, da den Forschern deutlich reinere humane Proteinmischungen zur Verfügung stehen. Darüber hinaus erleichtern Seppro^®-Säulen Proteomanalysen am Plasmaproteom und sorgen für reinere Ergebnisse.

Abgereicherte Proteine:

Albumin
IgG
α1-Antitrypsin
IgA
IgM
Transferrin
Haptoglobin
α2-Macroglobulin
Fibrinogen
Komplement C3
α1-saures Glykoprotein (Orosomucoid)
HDL (Apolipoproteine A-I und A-II)
LDL (vorwiegend Apolipoprotein B)

Anwendung

Seppro^® IgY14, LC2 (2 mL bed volume columns) is designed for use as a plasma/serum protein removal tool base upon immune affinity removal of 14 highly abundant proteins (HAP) from human plasma protein mixtures via liquid chromatography. Seppro^® IgY14 is used to support the analysis of low abundance human serum/plasma proteins.

Lagerung und Haltbarkeit

Store the column at 2–8 °C. After use, equilibrate the column with 1´ Dilution Buffer containing 0.02% sodium azide and store the column at 2–8 °C with the end-caps tightly sealed. Do Not Freeze the column.

Sonstige Hinweise

If the protein concentration of the sample is unknown and the total serum protein level is potentially elevated, a reduction of the serum load to 24.0 ml is recommended for initial study to avoid potential abundant protein bleed through.

Rechtliche Hinweise

Seppro is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit-Komponenten auch einzeln erhältlich

• S4199 Seppro^® Dilution buffer
• S4324 Seppro^® Stripping Buffer
• S4449 Seppro^® Neutralization Buffer
• CLS8163 Corning^® Costar^® Spin-X^® centrifuge tube filters, cellulose acetate membrane, pore size 0.45 μm, non-sterile

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids