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H7782

Sigma-Aldrich

Hybridization solution

Synonym(e):

Hyb solution, Hybridization solution for in situ hybridization, Ready-to-use hybridization solution

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About This Item

EG-Nummer:
200-842-0
UNSPSC-Code:
12161503
NACRES:
NA.32

Allgemeine Beschreibung

In situ hybridization assays detect a specific nucleic acid sequence of a gene at the cellular level. Hybridization solution is used to enhance hybrid formation between the DNA probe and the target mRNA on tissue sections. It may be used for reconstituting and/or diluting the probe for in situ hybridization experiments, or as a negative control (without probe).
Ready-to-use hybridization solution optimized for in situ hybridization (ISH) procedures.

Anwendung

Hybridization solution has been used in:
  • fluorescent in situ hybridization (FISH) to perform probe annealing
  • in situ hybridization (ISH) to dilute probe for the detection of amastigote forms of Leishmania infantum
  • FISH analysis to hybridize probes

Piktogramme

Health hazard
GHS08

Signalwort

Danger

Gefahreneinstufungen

Carc. 2 - Repr. 1B - STOT RE 2 Oral

Zielorgane

Blood

Lagerklassenschlüssel

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

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SSC-Puffer 20×-Konzentrat for Northern and Southern blotting, solution

Sigma-Aldrich

S6639

SSC-Puffer 20×-Konzentrat

DIG Easy Hyb™ suitable for hybridization, solution

Roche

11603558001

DIG Easy Hyb™

Block-Reagenz For nucleic acid hybridization and detection

Roche

11096176001

Block-Reagenz

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Taguchi T, et al.
Chromosome Analysis Protocols, 16, 33-38 (2013)
Melanie J Harriff et al.
Journal of bacteriology, 191(4), 1132-1142 (2008-12-09)
The Mycobacterium avium complex (MAC) is an important group of opportunistic pathogens for birds, cattle, swine, and immunosuppressed humans. Although invasion of epithelial cells lining the intestine is the chief point of entry for these organisms, little is known about
Ying Lv et al.
Journal of the American Heart Association, 8(2), e010456-e010456 (2019-01-15)
Background Intrauterine growth retardation ( IUGR ) is related to pulmonary artery hypertension in adults, and mi croRNA -206 (miR-206) is proposed to affect the proliferation and apoptosis of pulmonary artery smooth muscle cells ( PASMC s) via post-transcriptional regulation.
Aurea V A da Silva et al.
Immunity, inflammation and disease, 8(2), 127-139 (2020-03-25)
In canine visceral leishmaniasis (CVL), lymphopenia, and the disorganization of lymphoid organs such as spleen and lymph nodes have been demonstrated. However, the involvement of thymus in CVL has not been evaluated so far. Herein, we investigated whether the thymus

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