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Merck

51412C

SAFC

Minimum Essential Medium Eagle

with Earle′s Balanced Salts, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(e):

MEM

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About This Item

UNSPSC-Code:
12352207
NACRES:
NA.75

Beschreibung

for research or for further manufacturing use

Sterilität

sterile-filtered

Form

liquid

Methode(n)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

Komponenten

L-glutamine: no
NaHCO3: 2200 mg/L
phenol red: 10.62 mg/L
Earle’s salts (5% CO2): yes

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Minimum Essential Medium (MEM) with Earle′s Balanced Salts was developed by Harry Eagle as a result of his studies to determine the essential nutritional requirements of mouse L cells and HeLa cells in culture. He demonstrated that these cells require 13 amino acids and seven vitamins to grow and reproduce in vitro. MEM is a modification of Eagle′s earlier medium Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. This medium has demonstrated the ability to support a variety of normal and transformed cells in culture and contains Earle′s Balanced Salts, which makes it suitable for use in atmospheres charged with CO2 gas.

Anwendung

Minimum essential medium (MEM) has been used as a growth medium for bone marrow mesenchymal stem cells (BMSCs) and neurons.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves


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