524751-M

Phosphodiesterase 7A1, Human, Recombinant, S. frugiperda

• Synonym(e): hPDE7A1
• UNSPSC-Code: 12352203

Eigenschaften

• Rekombinant: expressed in baculovirus infected Sf21 cells
• Qualitätsniveau: 100
• Form: liquid
• Spezifische Aktivität: ≥0.5 U/μL
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Full-length, recombinant, human phosphodiesterdase 7A1 (PDE7A1) expressed in S. frugiperda insect cells using a baculovirus expression system. PDE7A1 is required for T-cell activation induced by the costimulation of CD3 and CD28 receptors. Increased PDE7A1 correlates with a decrease in cAMP, increased IL-2, and increased cell proliferation. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.

Warnhinweis

Toxicity: Standard Handling (A)

Einheitendefinition

One unit is defined as the amount of enzyme that is required to convert 1 pmol cAMP to AMP per min at 30°C, pH 7.5.

Physikalische Form

In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.

Rekonstituierung

Following initial thaw, aliquot and freeze (-70°C).

Sonstige Hinweise

Han, P., et al. 2006. J. Biol. Chem.281, 15050.
Lugnier, C. 2006. Pharmacol Ther.109, 366.
Bloom, T.J., and Beavo, J.A. 1996. Proc. Natl. Acad. Sci. U S A93, 14188.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable