07-1507

Anti-phospho-Mypt1 (Ser507) Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, Protein phosphatase myosin-binding subunit
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: mouse
• Speziesreaktivität (Voraussage durch Homologie): human (based on 100% sequence homology), chicken (based on 100% sequence homology), bovine (based on 100% sequence homology)
• Methode(n): western blot: suitable
• NCBI-Hinterlegungsnummer: NP_002471
• UniProt-Hinterlegungsnummer: O14974
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: phosphorylation (pSer507)
• Angaben zum Gen: human ... PPP1R12A(4659)

Beschreibung

Allgemeine Beschreibung

Myosin phosphatase targeting subunit 1 (MYPT1) is a regulatory targeting subunit that is thought to be responsible for targeting the holoenzyme to the proper positions in muscle and moving PP1 to myosin. MYPT1 is the major subunit involved in myosin light chain phosphatase (MLCP). It is also essential for vasodilation caused by nitric oxide (NO). A decrease in MYPT1 has been shown to play a role in increased tolerance of NO in pulmonary arteries.

Spezifität

Other homologies: Rat (89% sequence homology).

This antibody recognizes Mypt1 phosphorylated at (Ser507).

Immunogen

Epitope: Phosphorylated (Ser507)

KLH-conjugated linear peptide corresponding to human Mypt1 phosphorylated at (Ser507).

Anwendung

Research Category
Zelluläre Signaltransduktion

Zellstruktur

Research Sub Category
Entwicklungsabhängige Signalübertragung

Muskelphysiologie

This Anti-phospho-Mypt1 (Ser507) Antibody is validated for use in WB for the detection of phospho-Mypt1 (Ser507).

Qualität

Evaluated by Western Blot in untreated and PMA-treated NIH/3T3 cell lysates.

Western Blot Analysis: 2 µg/mL of this antibody detected Mypt1 in 10 µg of untreated and PMA-treated NIH/3T3 cell lysates.

Zielbeschreibung

~120 kDa observed.
Uniprot gives a calculated molecular weight of 115 kDa, which can be observed at higher molecular weights due to hyperphosphorylation. Uncharacterized bands appear at ~30 kDa and ~130 kDa in some lysates.

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) and 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Untreated and PMA-treated NIH/3T3 cell lysates

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable