- Composites of gellan gum hydrogel enzymatically mineralized with calcium-zinc phosphate for bone regeneration with antibacterial activity.
Composites of gellan gum hydrogel enzymatically mineralized with calcium-zinc phosphate for bone regeneration with antibacterial activity.
Journal of tissue engineering and regenerative medicine (2015-07-16)
Timothy E L Douglas, Magdalena Pilarz, Marco Lopez-Heredia, Gilles Brackman, David Schaubroeck, Lieve Balcaen, Vitaliy Bliznuk, Peter Dubruel, Christine Knabe-Ducheyne, Frank Vanhaecke, Tom Coenye, Elzbieta Pamula
PMID26174042
ABSTRACT
Gellan gum hydrogels functionalized with alkaline phosphatase were enzymatically mineralized with phosphates in mineralization medium containing calcium (Ca) and zinc (Zn) to improve their suitability as biomaterials for bone regeneration. The aims of the study were to endow mineralized hydrogels with antibacterial activity by incorporation of Zn in the inorganic phase, and to investigate the effect of Zn incorporation on the amount and type of mineral formed, the compressive modulus of the mineralized hydrogels and on their ability to support adhesion and growth of MC3T3-E1 osteoblast-like cells. Mineralization medium contained glycerophosphate (0.05 m) and three different molar Ca:Zn ratios, 0.05:0, 0.04:0.01 and 0.025:0.025 (all mol/dm
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