Skip to Content
Merck
  • Transient Treg-cell depletion in adult mice results in persistent self-reactive CD4(+) T-cell responses.

Transient Treg-cell depletion in adult mice results in persistent self-reactive CD4(+) T-cell responses.

European journal of immunology (2014-09-19)
Sofia N Nyström, Dorothée Bourges, Sarah Garry, Ellen M Ross, Ian R van Driel, Paul A Gleeson
ABSTRACT

Depletion of Foxp3(+) CD4(+) regulatory T cells (Treg) in adults results in chronic inflammation and autoimmune disease. However, the impact of transient Treg-cell depletion on self-reactive responses is poorly defined. Here, we studied the effect of transient depletion of Treg cells on CD4(+) T-cell responses to endogenous self-antigens. Short-term ablation of Treg cells in mice resulted in rapid activation of CD4(+) T cells, increased percentage of IFN-γ(+) and Th17 cells in lymphoid organs, and development of autoimmune gastritis. To track self-reactive responses, we analyzed the activation of naïve gastric-specific CD4(+) T cells. There was a dramatic increase in proliferation and acquisition of effector function of gastric-specific T cells in the stomach draining LNs of Treg-cell-depleted mice, compared with untreated mice, either during Treg-cell depletion or after Treg-cell reconstitution. Moreover, the hyperproliferation of gastric-specific T cells in the Treg-cell-ablated mice was predominantly antigen-dependent. Transient depletion of Treg cells resulted in a shift in the ratio of peripheral:thymic Treg cells in the reemerged Treg-cell population, indicating an altered composition of Treg cells. These findings indicate that transient Treg-cell depletion results in ongoing antigen-driven self-reactive T-cell responses and emphasize the continual requirement for an intact Treg-cell population.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
1-Decanethiol, 96%
Sigma-Aldrich
1-Decanethiol, 99%