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  • Biocompatibility and setting time of CPM-MTA and white Portland cement clinker with or without calcium sulfate.

Biocompatibility and setting time of CPM-MTA and white Portland cement clinker with or without calcium sulfate.

Journal of applied oral science : revista FOB (2013-04-06)
Clovis Monteiro Bramante, Marcia Magro Kato, Gerson Francisco de Assis, Marco Antonio Hungaro Duarte, Norberti Bernardineli, Ivaldo Gomes de Moraes, Roberto Brandão Garcia, Ronald Ordinola-Zapata, Alexandre Silva Bramante
ABSTRACT

To evaluate the biocompatibility and the setting time of Portland cement clinker with or without 2% or 5% calcium sulfate and MTA-CPM. Twenty-four mice (Rattus norvegicus) received subcutaneously polyethylene tubes filled with Portland cement clinker with or without 2% or 5% calcium sulfate and MTA. After 15, 30 and 60 days of implantation, the animals were killed and specimens were prepared for microscopic analysis. For evaluation of the setting time, each material was analyzed using Gilmore needles weighing 113.5 g and 456.5 g, according to the ASTM specification Number C266-08 guideline. Data were analyzed by ANOVA and Tukey's test for setting time and Kruskal-Wallis and Dunn test for biocompatibility at 5% significance level. Histologic observation showed no statistically significant difference of biocompatibility (p>0.05) among the materials in the subcutaneous tissues. For the setting time, clinker without calcium sulfate showed the shortest initial and final setting times (6.18 s/21.48 s), followed by clinker with 2% calcium sulfate (9.22 s/25.33 s), clinker with 5% calcium sulfate (10.06 s/42.46 s) and MTA (15.01 s/42.46 s). All the tested materials showed biocompatibility and the calcium sulfate absence shortened the initial and final setting times of the white Portland cement clinker.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Calcium sulfate, ≥99.99% trace metals basis
Sigma-Aldrich
Calcium sulfate, −325 mesh, 99%
Sigma-Aldrich
Calcium sulfate dihydrate, puriss., meets analytical specification of NF, E 516, 99.0-101.0% (based on anhydrous substance)
Sigma-Aldrich
Calcium sulfate dihydrate, ACS reagent, 98%
Sigma-Aldrich
Calcium sulfate dihydrate, ReagentPlus®, ≥99%
Sigma-Aldrich
Drierite, with indicator, 10-20 mesh
Sigma-Aldrich
Drierite, without indicator, 4 mesh
Sigma-Aldrich
Drierite, with indicator, 8 mesh
Sigma-Aldrich
Drierite, without indicator, 8 mesh
Sigma-Aldrich
Drierite, with indicator, 4 mesh
Sigma-Aldrich
Calcium sulfate hemihydrate, purum, ≥97.0%
Sigma-Aldrich
Drierite, with indicator, 6 mesh