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  • [Effect of aflatoxin G1 on the expression of HLA- I molecule in human esophageal epithelial cells].

[Effect of aflatoxin G1 on the expression of HLA- I molecule in human esophageal epithelial cells].

Wei sheng yan jiu = Journal of hygiene research (2011-03-26)
Zengning Li, Jinfeng Cui, Xianghong Zhang, Junling Wang
ABSTRACT

To explore the effect of AFG1 on the expression of human leukocyte antigen (HLA-I) molecule in human esophageal epithelial cells. Western Blot and RT-PCT analysis was used to explore the effect of AFG1 on the antigen presenting function of primary cultured esophageal epithelial cells. The protein expression of HLA-ABC was significantly decreased in the groups treated with 100, 1000 and 2000 microg/L AFG1 as compared with the solvent control group (P < 0.05). Within the range of 100 - 2000 microg/L AFG1, the protein expression level of HLA-ABC of esophageal epithelial cells decreased gradually, and showed significant negative correlation (r = -0.921, n = 3, P < 0.01). The effect of AFG1 on the expression of HLA-ABC mRNA was further confirmed by RT-PCR analysis. The results showed that after treated with 100 microg/L, 1000 microg/L and 2000 microg/L AFG1, the expression of HLA-A mRNA was decreased significantly as compared with the solvent control (P < 0.05), and the expression of HLA-B mRNA was decreased in the 2000 microg/L AFG1 treated group (P < 0.05), while there was no effect on the expression of HLA-C mRNA. The expression of HLA- I molecule in human esophageal epithelial cells could be inhibited by AFG1 treatment.

MATERIALS
Product Number
Brand
Product Description

Supelco
Aflatoxin G1
Sigma-Aldrich
Aflatoxin G1, from Aspergillus flavus
Aflatoxin G1 solution, 3.78 μg/g in acetonitrile, ERM®, certified reference material
Supelco
Aflatoxin G1 solution, 2 μg/mL in acetonitrile, analytical standard