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Key Documents

BHK 21 CL13(IZS)

93120815, hamster kidney, Fibroblast

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About This Item

UNSPSC Code:
41106514

product name

BHK 21 CL13(IZS), 93120815

biological source

hamster kidney

growth mode

Adherent

karyotype

2n = 44

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Hamster Syrian kidney

Cell Line Description

Susceptible to a range of viruses including adenovirus 25, vaccinia, herpes simplex, reovirus 3, vesicular stomatitis, rubella, pseudorabies.

Application

Virus studies

Culture Medium

GMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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