A2582

Anti-Thioredoxin–Agarose antibody produced in rabbit

IgG fraction of antiserum, PBS suspension

• Synonym(s): Anti-Thioredoxin
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: rabbit
• conjugate: agarose conjugate
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: PBS suspension
• mol wt: antigen 11.7 kDa
• species reactivity: E. coli
• analyte chemical class(es): proteins (thioredoxin)
• technique(s): affinity chromatography: suitable; immunoprecipitation (IP): suitable
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Thioredoxin is a small electron transport protein. The thioredoxin system consists of thioredoxin, thioredoxin-reductase and NADPH. Thioredoxin obtained from E. coli is made up of a single polypeptide chain of 108 amino acids. It has a molecular weight of 11,700. The protein contains no prosthetic group or bound metals.

Specificity

Specific for natural E. coli and recombinant thioredoxin. It may be used to identify and purify the expression of thioredoxin fusion proteins.

Immunogen

recombinant E. coli thioredoxin.

Application

Anti-Thioredoxin−Agarose antibody produced in rabbit has been used for immunoaffinity purification and immunoprecipitation of thioredoxin from bacterial lysates.

Immunofluorescence was carried out on the cerivcal cancer cell lines SiHa, CaSki, and HeLa using an antibody against the redox proteinThioredoxin.

Biochem/physiol Actions

Thioredoxin is a 11.7kDa electron transport protein that functions as a hydrogen donor during the enzymatic reduction of ribonucleotides to yield deoxyribonucleotides. The thioredoxin system is also involved in the enzymatic reductions of methionine sulfoxide and sulfate. The gene encoding E. coli thioredoxin has been cloned into several plasmids and serves as a fusion partner for the expression of other cloned genes. Hence, the system is useful for the production of soluble fusion proteins in the E. coli cytoplasm. Furthermore, in several instances, these fusion proteins fold correctly and thus display full biological activity.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Prepared using antibody coupled to cyanogen bromide activated agarose at 5 mg antibody per mL resin volume.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.