MABN70
Anti-Slo1 Antibody, clone L6/60
clone L6/60, from mouse
Synonym(s):
potassium large conductance calcium-activated channel, subfamily M, alpha member 1, Slo homolog, stretch-activated Kca channel, Maxi K channel, Drosophila slowpoke-like, Calcium-activated potassium channel, subfamily M subunit alpha-1, Slowpoke homolog,
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
L6/60, monoclonal
species reactivity
rat
species reactivity (predicted by homology)
mouse (immunogen homology)
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... KCNMA1(3778)
General description
Slo1 (Calcium-activated potassium channel subunit alpha-1) may not only be activated by membrane depolarization but may also be by an increase in cytosolic Ca2+. The concentration of cytosolic Mg2+ also activates this potassium channel. Found to play a part in the repolarization of the membrane potential due to the fact that it diminishes the excitatory voltage in the cell.
Specificity
This antibody recognizes S9-S10 segments of Slo1.
Immunogen
Epitope: S9-S10 segments
Recombinant protein corresponding to the S9-S10 segments of mouse Slo1.
Application
Detect Slo1 using this Anti-Slo1 Antibody, clone L6/60 validated for use in IH, WB & IC.
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Slo1 in rat cerebellum tissue.
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (Misonou, H., et al. (2006). J Comp Neurol. 496(3):289–302.)
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (Misonou, H., et al. (2006). J Comp Neurol. 496(3):289–302.)
Research Category
Neuroscience
Neuroscience
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Quality
Evaluated by Western Blot in rat brain membrane tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Slo1 on 10 µg of rat brain membrane tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Slo1 on 10 µg of rat brain membrane tissue lysate.
Target description
~100 kDa observed
Physical form
Format: Purified
Protein G
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat brain membrane tissue lysate
Rat brain membrane tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Andrew J Nickerson et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(5), e21606-e21606 (2021-04-29)
Na+ -K+ -2Cl- cotransporter-1 (NKCC1) facilitates basolateral K+ and Cl- uptake, supporting their efflux across mucosal membranes of colonic epithelial cells. NKCC1 activity has also been shown to be critical for electrogenic K+ secretion induced by aldosterone, which is known
Boxing Li et al.
Nature neuroscience, 17(8), 1055-1063 (2014-06-24)
Ion channels are essential for the regulation of neuronal functions. The significance of plasma membrane, mitochondrial, endoplasmic reticulum and lysosomal ion channels in the regulation of Ca(2+) is well established. In contrast, surprisingly little is known about the function of
Agbonlahor Okhuarobo et al.
Molecular psychiatry (2023-12-23)
Large conductance potassium (BK) channels are among the most sensitive molecular targets of ethanol and genetic variations in the channel-forming α subunit have been nominally associated with alcohol use disorders. However, whether the action of ethanol at BK α influences
Oscar Vivas et al.
eLife, 6 (2017-07-01)
CaV-channel dependent activation of BK channels is critical for feedback control of both calcium influx and cell excitability. Here we addressed the functional and spatial interaction between BK and CaV1.3 channels, unique CaV1 channels that activate at low voltages. We
Shangru Lyu et al.
Neuroscience, 505, 78-90 (2022-10-17)
Genetic analyses have linked BTBD9 to restless legs syndrome (RLS) and sleep regulation. Btbd9 knockout mice show RLS-like motor restlessness. Previously, we found hyperactivity of cerebellar Purkinje cells (PCs) in Btbd9 knockout mice, which may contribute to the motor restlessness
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