HPLC Analysis of MAb 016 on Proteomix^® SCX NP5 versus Competitive SCX using a Salt Gradient

column

Proteomix SCX-NP5, 25 cm x 4.6 mm I.D. (PEEK hardware), 5 μm particles (Z777164)

mobile phase

(A) 20 mM phosphate buffer, pH 7.5; (B) A + 1 M NaCl

gradient

0 to 6% B in 30 min

flow rate

0.8 mL/min

detector

UV, 280 nm

injection

4 μL

sample

MAb 016 (11.8 mg/mL)