SRP6464

ApoJ/Clusterin human

recombinant, expressed in HEK 293 cells, ≥92% (SDS-PAGE)

• Synonyme(s) : AAG4, Apolipoprotein J, CLI, CLU, KUB1, MGC24903, SGP-2, SGP2, SP-40, TRPM-2, TRPM2
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in HEK 293 cells
• Étiquette/Marqueur: 6-His tagged (C-terminus)
• Pureté: ≥92% (SDS-PAGE)
• Forme: lyophilized
• Poids mol.: calculated mol wt 50.9 kDa; observed mol wt 34-37 kDa by SDS-PAGE
• Conditionnement: pkg of 10 μg; pkg of 50 μg
• Impuretés: <1 EU/μg endotoxin (LAL test)
• Numéro d'accès UniProt: P10909
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... CLU(1191)

Description

Description générale

Asp23-Glu449, α chain 24.5kDa +β chain 27_kDa.The recombinant rh CLUS / Clusterin (Asp23-Glu 449) was cleaved into α chain and β chain, which form a heterodimer linked by disulfide bonds.

DTT reduced. corresponding to the cleaved β chain, andβ chain respectively.

Native Apolipoprotein J (ApoJ), also named Clusterin, is a heavily glycosylated, 75-80 kDa disulfide-linked heterodimeric protein. Despite being cloned since 1989, no genuine function has been attributed to ApoJ so far. The protein has been reportedly implicated in several diverse physiological processes such as sperm maturation, lipid transportation, complement inhibition, tissue remodeling, and membrane recycling, cell-cell and cell-substratum interactions, stabilization of stressed proteins in a folding-competent state and promotion or inhibition of apoptosis. ApoJ gene is differentially regulated by cytokines, growth factors and stress-inducing agents. Clusterin is up- or down regulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.

Forme physique

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable