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Key Documents

SRP0117

Sigma-Aldrich

Sirtuin 3 human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonyme(s) :

Mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase, SIR2L3, SIRT3, Silent mating type information regulation 2, sir2-like 3

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in E. coli

Pureté

≥70% (SDS-PAGE)

Forme

aqueous solution

Poids mol.

59 kDa

Conditionnement

pkg of 100 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Concentration

>0.02 mg/mL

Numéro d'accès NCBI

NM_012239

Numéro d'accès UniProt

Q9NTG7

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... SIRT3(23410)

Description générale

Sirtuin 3 (SIRT3) is a member of a family of NAD+-dependent protein deacetylases called sirtuins (SIRTs). In mammals, there are seven types of SIRTs (SIRT1 to 7). SIRT 3 is present in mitochondrial matrix.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Actions biochimiques/physiologiques

Sirtuin 3 (SIRT3) is thought to be involved in metabolism as its expression is suppressed in muscles of diabetic animals and increased in white and brown adipose tissues during calorie restriction (CR). This protein modulates the degree of acetylation and activity of acetyl-coenzyme A synthetase 2, an enzyme thought to be involved in energy production during starvation conditions in mammals. Up-regulation of this gene influences the expression of genes involved in mitochondrial function. During fasting, SIRT3 controls mitochondrial intermediary metabolism and fatty-acid use by deacetylating long-chain acyl coenzyme A dehydrogenase (LCAD). It is an essential modulator of basal ATP, and therefore, overall energy homeostasis.

Définition de l'unité

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation.
Hirschey MD, et al.
Nature, 464(7285), 121-125 (2010)
Virtual Screening Combined with Enzymatic Assays to Guide the Discovery of Novel SIRT2 Inhibitors.
Scarano, et al.
International Journal of Molecular Sciences, 24 (2023)
A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis.
Ahn BH
Proceedings of the National Academy of Sciences of the USA, 105(38), 14447-14452 (2008)
David B Lombard et al.
Molecular and cellular biology, 27(24), 8807-8814 (2007-10-10)
Homologs of the Saccharomyces cerevisiae Sir2 protein, sirtuins, promote longevity in many organisms. Studies of the sirtuin SIRT3 have so far been limited to cell culture systems. Here, we investigate the localization and function of SIRT3 in vivo. We show
L Pacella-Ince et al.
Human reproduction (Oxford, England), 29(7), 1490-1499 (2014-04-29)
Is the activity of sirtuin 3 (SIRT3) altered in granulosa and cumulus cells from young women with reduced ovarian reserve or women of advanced maternal age? SIRT3 mRNA and active protein in granulosa and cumulus cells were decreased in women
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