SRP0106

HDAC-5 human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• Synonyme(s) : HD5, Histone deacetylase 5, NY-CO-9
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in baculovirus infected insect cells
• Pureté: ≥80% (SDS-PAGE)
• Forme: aqueous solution
• Puissance: ≥1 units/mg
• Poids mol.: 51 kDa
• Conditionnement: pkg of 10 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: NM_001015053
• Numéro d'accès UniProt: Q9UQL6
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... HDAC5(10014)

Description

Description générale

Human HDAC5, catalytic domain, GenBank Accession No. NM_001015053, amino acid 657-1123 with C-terminal His tag, MW = 51 kDa, expressed in baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Définition de l'unité

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable