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Key Documents

SAB5600227

Sigma-Aldrich

Anti-His-Tag antibody, Rabbit monoclonal

recombinant, expressed in HEK 293 cells, clone RM146, purified immunoglobulin

Synonyme(s) :

10 His epitope tag, 6 His epitope tag, HHHHHH tag, Polyhistidine Tag

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Produit recombinant

expressed in HEK 293 cells

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

RM146, monoclonal
recombinant monoclonal

Forme

buffered aqueous glycerol solution

Espèces réactives

all

Technique(s)

flow cytometry: 0.5-2 μg/mL
immunocytochemistry: 0.5-2 μg/mL
immunohistochemistry: 0.1—1 μg/mL
immunoprecipitation (IP): 0.5-2 μg/mL
western blot: 0.1— 1 μg/mL

Isotype

IgG

Numéro d'accès NCBI

8350

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Spécificité

This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.

Immunogène

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Forme physique

Solution in phosphate buffered saline containing 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Human inhalable antibody fragments neutralizing SARS-CoV-2 variants for COVID-19 therapy.
Minenkova, et al.
Molecular Therapy, 30, 1979-1993 (2022)
Tarah Elizabeth Trebino et al.
eLife, 12 (2023-12-27)
BRAF is a key member in the MAPK signaling pathway essential for cell growth, proliferation, and differentiation. Mutant BRAF is often the underlying cause of various types of cancer and mutant RAS, the upstream regulator of BRAF, is a driver
Harman Malhi et al.
Cell reports. Medicine, 3(6), 100658-100658 (2022-06-16)
Epstein-Barr virus (EBV) is a cancer-associated pathogen responsible for 165,000 deaths annually. EBV is also the etiological agent of infectious mononucleosis and is linked to multiple sclerosis and rheumatoid arthritis. Thus, an EBV vaccine would have a significant global health
Kristina R Edwards et al.
NPJ vaccines, 9(1), 120-120 (2024-06-27)
Epstein-Barr virus (EBV) is associated with several malignancies, neurodegenerative disorders and is the causative agent of infectious mononucleosis. A vaccine that prevents EBV-driven morbidity and mortality remains an unmet need. EBV is orally transmitted, infecting both B cells and epithelial
Kentaro Yumoto et al.
Antioxidants (Basel, Switzerland), 9(11) (2020-10-31)
Emphysema, a type of lung-destroying condition associated with chronic obstructive pulmonary disease (COPD), is an inflammatory lung disease mainly due to cigarette smoke exposure. As there is no curative therapy, prevention should be considered first by cessation of smoking to
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