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Key Documents

SAB4700215

Sigma-Aldrich

Monoclonal Anti-CD63 antibody produced in mouse

clone MEM-259, purified immunoglobulin, buffered aqueous solution

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

MEM-259, monoclonal

Forme

buffered aqueous solution

Espèces réactives

human

Concentration

1 mg/mL

Technique(s)

flow cytometry: suitable

Isotype

IgG1

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CD63(967)

Description générale

Cluster of differentiation 63 (CD63), also known as melanoma 1 antigen, is encoded by the gene mapped to human chromosome 12q13.2. The encoded protein belongs to the transmembrane 4 superfamily (TM4SF). CD63 is an integral membrane glycoprotein expressed in late endosomes and lysosomes and is also present in the platelet dense granule membrane.
The antibody MEM-259 reacts with CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.

Immunogène

HPB-ALL T cell line

Application

Monoclonal Anti-CD63 antibody produced in mouse has been used in flow cytometry and confocal imaging.
The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 2 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Actions biochimiques/physiologiques

Cluster of differentiation 63 (CD63) is one of the platelet activation marker, which plays a vital role in platelet aggregation, adhesion to collagen, uptake of oxidized low-density lipoprotein (LDL) in vitro and regulation of angiogenesis. Inadequate expression of CD63 results in the autosomal recessive inherited disorder, Hermansky-Pudlak syndrome. CD63 is associated with various cellular functions, such as cellular adhesion, cell differentiation, migration, carcinogenesis and tumor progression. CD63 is highly expressed in the initial stage of Merkel cell carcinoma and decreased in later stages; therefore, CD63 is considered to be a potential prognostic factor for this malignancy.

Caractéristiques et avantages

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forme physique

Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Tra-Ly Nguyen et al.
Cancer research, 78(18), 5384-5397 (2018-07-29)
The mTOR is a central regulator of cell growth and is highly activated in cancer cells to allow rapid tumor growth. The use of mTOR inhibitors as anticancer therapy has been approved for some types of tumors, albeit with modest
Jayanthi Lea et al.
Oncotarget, 8(9), 14395-14407 (2017-01-26)
There are no suitable screening modalities for ovarian carcinomas (OC) and repeated imaging and CA-125 levels are often needed to triage equivocal ovarian masses. Definitive diagnosis of malignancy, however, can only be established by histologic confirmation. Thus, the ability to
Gene expression signature associated with BRAF mutations in human primary cutaneous melanomas
Kannengiesser C
Molecular Oncology, 1, 425-430 (2008)
Detection of phosphatidylserine-positive exosomes as a diagnostic marker for ovarian malignancies: a proof of concept study
Lea J, et.al.
Oncotarget, 8, 14395-14407 (2017)
The protein CD63 is in platelet dense granules, is deficient in a patient with Hermansky-Pudlak syndrome, and appears identical to granulophysin.
Nishibori M
The Journal of Clinical Investigation, 91, 1775-1782 (1993)

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