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SAB4200541

Sigma-Aldrich

Anti-ADAR1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonyme(s) :

Anti-136 kDa double-stranded RNA-binding protein, Anti-DRADA, Anti-DSH, Anti-G1P1, Anti-IFI-4, Anti-IFI4, Anti-K88DSRBP, Anti-P136, Anti-adenosine deaminase acting on RNA 1-A, Anti-adenosine deaminase, RNA-specific, Anti-double-stranded RNA-specific adenosine deaminase, Anti-interferon-induced protein 4

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~110 kDa

Espèces réactives

human

Concentration

~1.0 mg/mL

Technique(s)

immunoprecipitation (IP): 10-20 μg using lysates of human HepG2 or HEK-293T cells.
indirect immunofluorescence: 5-10 μg/mL using human HepG2 cells.
western blot: 2-4 μg/mL using whole extracts of human HEK-293T cells.

Numéro d'accès UniProt

P55265

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

bovine ... ADAR(505134)
human ... ADAR(103)
mouse ... Adar(56417)
rat ... Adar(81635)

Catégories apparentées

Description générale

Adenosine deaminase that acts in RNA (ADAR) exists in two isoforms namely, ADAR1 and ADAR2, in mammals. Both the isoforms are expressed in variety of tissues but in general, the pre-mRNA being edited represents receptors of the central nervous systems. ADAR1 has several isoforms which include the full length ADAR1 (150 kDa) and two functionally active short isoforms (80 and 110 kDa).

Immunogène

synthetic peptide corresponding to an internal s of human ADAR1, conjugated to KLH. The corresponding sequence is identical in mouse, rat, bovine, pig and monkey.

Application

Anti-ADAR1 antibody produced in rabbit has been used in
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Actions biochimiques/physiologiques

Adenosine deaminase that acts in RNA 1 (ADAR1) play an important role in A-to-I RNA editing. In addition, it is also implicated in differentiating cells of embryo and adult tissues, facilitating cell survival and permitting their further differentiation and maturation. Its role in embryonic development is specifically observed within the hematopoietic lineage as well as in adult hematopoietic progenitor cells (HPCs). ADAR1 plays a key role in hematopoietic stem cell maintenance. It also block interferon signaling, that might help organisms to fight against the harmful effects of interferon activation linked with various diseases, such as chronic inflammation, autoimmune disorders and cancer.

Forme physique

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Guermarie Velazquez-Torres et al.
Nature communications, 9(1), 461-461 (2018-02-02)
Previously we have reported that metastatic melanoma cell lines and tumor specimens have reduced expression of ADAR1 and consequently are impaired in their ability to perform A-to-I microRNA (miRNA) editing. The effects of A-to-I miRNAs editing on melanoma growth and
A-to-I miR-378a-3p editing can prevent melanoma progression via regulation of PARVA expression
Velazquez-Torres G, et al.
Nature Communications, 9(1), 461-461 (2018)
RNA editing catalyzed by ADAR1 and its function in mammalian cells
Wang Q
Biochemistry (Moscow), 76(8), 900-900 (2011)
ADAR1 is essential for the maintenance of hematopoiesis and suppression of interferon signaling
Hartner JC, et al.
Nature Immunology, 10(1), 109-109 (2009)
Intracellular localization of differentially regulated RNA-specific adenosine deaminase isoforms in inflammation
Yang JH, et al.
The Journal of biological chemistry, 278(46), 45833-45842 (2003)
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