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Key Documents

S3396

Sigma-Aldrich

Monoclonal Anti-Spectrin (α and β) antibody produced in mouse

clone SB-SP1, ascites fluid

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

SB-SP1, monoclonal

Contient

15 mM sodium azide

Espèces réactives

human

Technique(s)

indirect immunofluorescence: suitable using erythrocyte smears
microarray: suitable
western blot: 1:500 using extract of human erythrocyte ghosts or purified human erythrocyte spectrin

Isotype

IgG1

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

Description générale

Monoclonal anti-Spectrin (α and β) (mouse IgG1 isotype) is produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Spectrin is a major constituent of the erythrocyte skeleton accounting for 25% of the total membrane protein and 75% of the cytoskeletal mass.
Spectrins are heterotetramers, that are composed of several αand β subunit isoforms. They are the constituents of G-protein-coupled receptor (GPCR) and the synaptic multiprotein complex. α-Spectrin has 20 spectrin repeats (α1-α20) and β-spectrin has 17 spectrin repeats (β1-β17). SPTA1 (spectrin α 1) is located on chromosome 1q21–q23 and SPTB (spectrin β) is located on chromosome 14q22–q23.2.

Spécificité

This antibody reacts specifically with the α and β chains of human erythrocyte spectrin.

Immunogène

human erythrocyte spectrin.

Application

Anti-Spectrin (α and β) antibody has been used in western blotting and immunostaining.
Monoclonal Anti-Spectrin (α and β) antibody produced in mouse has been used in flow cytometry analysis and confocal microscopy.

Actions biochimiques/physiologiques

Spectrins are multifunctional proteins, that modulates the cell morphology and mechanical properties. Spectrins also participates in the cell cycle by controlling the upregulation of membrane receptors. α- and β-spectrins are essential for the development of nervous system.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Membrane protein carbonylation in non-leukodepleted CPDA-preserved red blood cells
Kriebardis AG, et al.
Blood Cells, Molecules and Diseases, 36(2), 279-282 (2006)
Specificity of commercial anti-spectrin antibody in the study of fungal and Oomycete spectrin: cross-reaction with proteins other than spectrin
Cotado-Sampayo M, et al.
Fungal genetics and biology : FG & B, 45(6), 1008-1015 (2008)
Spectrin labeling during oogenesis in zebrafish (Danio rerio)
Okuthe GE and Fabian BC
Acta Histochemica, 114(2), 177-181 (2012)
Protein area occupancy at the center of the red blood cell membrane
Dupuy AD and Engelman DM
Proceedings of the National Academy of Sciences of the USA, 105(8), 2848-2852 (2008)
Indicators of erythrocyte damage after microwave warming of packed red blood cells
Hirsch J, et al.
Clinical Chemistry, 49(5), 792-799 (2003)

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