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Key Documents

R7020

Sigma-Aldrich

Transcript RNA Markers 0.2-10 kb

for RNA electrophoresis

Synonyme(s) :

RNA agarose gel electrophoresis ladder, RNA agarose gel electrophoresis marker, northern blot ladder, northern blot marker, ssRNA ladder, ssRNA marker, tRNA ladder, tRNA marker

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About This Item

Code UNSPSC :
41105337
Nomenclature NACRES :
NA.25

Qualité

for molecular biology

Niveau de qualité

Forme

liquid

Utilisation

25 uses

Adéquation

suitable for electrophoresis (RNA)

Température de stockage

−70°C

Description générale

Sigma′s Transcript RNA Marker (0.2 – 10 kb) contains 9 RNA transcripts from 200 – 10,000 bases. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Application

Suitable for size determination of small RNA using native or denaturing agarose gel electrophoresis.
Transcript RNA Markers 0.2-10 kb has been used as a molecular weight marker for northern blot hybridization.

Composants

Sigma′s Transcript RNA Marker (0.2 – 10 kb) is provided in a gel-loading solution with 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Autres remarques

Contains nine defined RNA transcripts, 200–10,000 nucleotides
For optimal resolution, the recommended agarose gel concentration is 1.0%.

Produit(s) apparenté(s)

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Articles

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

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