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MBD0018

Sigma-Aldrich

Microbial DNA standard from Bacillus subtilis

Suitable for PCR, Sequencing and NGS, 10 ng/μL

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About This Item

Code UNSPSC :
41105500
Nomenclature NACRES :
NA.24

Niveau de qualité

Forme

liquid

Concentration

10 ng/μL

Technique(s)

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

Conditions d'expédition

ambient

Température de stockage

−20°C

Description générale

Microbial DNA standard from Bacillus subtilis (B. subtilis) is a genomic DNA microbial standard that can serve as a standard for benchmarking the performance, along the workflow of microbiomics or meta-genomics analyses. It can also be used as a tool to increase reproducibility and allow comparison of results obtained by different labs. Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses. Bacillus subtilis is a gram positive, aerobic bacterium, rod-shaped, endospore-forming, catalase-positive. It is traditionally considered as a soil microorganism. Bacillus subtilis is genetically well characterized and serves as a model organism for important gram-positive pathogens such as Bacillus anthracis, Staphylococcus aureus etc. B. subtilis possess an extreme adaptation ability to various environmental stress conditions by modifying its motility, uptake of exogenous DNA, biofilm formation and sporulation. Recent studies have identified and characterized B. subtilis isolates from human gastrointestinal track suggesting B. subtilis as a gut commensal. Moreover, B. subtilis presence in the gut was found to correlate with probiotic effects such as anti-diarrhea effect, anti-microbial effect, immune-stimulatory effect, competition with pathogens, normal flora growth stimulation and prevention of intestinal inflammation. B. subtilis beneficial effect on gut inflammation may also suggest it as potential probiotic treatment for inflammatory bowel diseases (IBD), including Crohn′s and ulcerative colitis. In addition, it was demonstrated that a peptide produced by B. subtilis possess antiviral effect against influenza virus.




Read here how to use our standards to ensure data integrity for your microbiome research.

Application

Suitable for Quantitative standard for PCR, Sequencing and NGS

Caractéristiques et avantages

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Forme physique

Liquid - The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0

Autres remarques

It is recommended to avoid freeze thaw cycles of this product.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Bacillus subtilis?The Representative of Gram-Positive Bacteria
The Flagellar World, 22-23 (2014)
Subti wiki-a database for the model organism Bacillus subtilis that links pathway, interaction and expression information
Michna RH, et al.
Nucleic Acids Research, 42, D692-D698 (2013)
Amelioration of the DSS-induced colitis in mice by pretreatment with 4, 4'-diaponeurosporene-producing Bacillus subtilis
Jing Y, et al.
Experimental and Therapeutic Medicine, 14(6), 6069-6073 (2017)

Articles

DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.

DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.

DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.

DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.

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