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Key Documents

MBD0005

Sigma-Aldrich

Microbial DNA standard from Salmonella enterica

Suitable for PCR, sequencing and NGS, 10 ng/μL

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About This Item

Code UNSPSC :
41105500
Nomenclature NACRES :
NA.24

Niveau de qualité

200

Forme

liquid

Concentration

10 ng/μL

Technique(s)

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

Conditions d'expédition

ambient

Température de stockage

−20°C

Description générale

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses.1 Salmonella enterica genomic DNA microbial standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.
Salmonella enterica serotype Typhimurium (also S. Typhimurium) is a rod-shaped gram-negative bacterium of the family Enterobacteriaceae. It is an important food-borne pathogen that in humans causes intestinal inflammation, diarrhea, and the presence of neutrophils in stool samples.2 Growth of S. enterica in the gut lumen causes depletion of the healthy microbial community.3 Infection with Salmonella can induce inflammation and increase the risk for inflammatory bowel disease (IBD)4

Read here how to use our standards to ensure data integrity for your microbiome research.

Application

Suitable for Quantitative standard for PCR, Sequencing and NGS

Caractéristiques et avantages

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Forme physique

Liquid -The genomic DNA is provided at ≥10 ng/μl concentration in TE buffer pH 8.0

Autres remarques

Strain: Salmonella enterica subsp. enterica serovar Typhimurium strain

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Fecal leukocytes in diarrheal illness.
J C Harris et al.
Annals of internal medicine, 76(5), 697-703 (1972-05-01)
Inna Sekirov et al.
Gut microbes, 1(1), 30-41 (2011-02-18)
Gastrointestinal infections involve an interactive tripartite relationship between the invading pathogen, the host, and the host's resident intestinal microbiota. To characterize the host inflammatory response and microbiota alterations during enteric salmonellosis, C57BL/6 mice were pre-treated with a low dose of
Kim O Gradel et al.
Gastroenterology, 137(2), 495-501 (2009-04-14)
Various commensal enteric and potentially pathogenic bacteria may be involved in the pathogenesis of inflammatory bowel diseases (IBD). We compared the risk of IBD between a cohort of patients with documented Salmonella or Campylobacter gastroenteritis and an age- and gender-matched
J Paul Brooks et al.
BMC microbiology, 15, 66-66 (2015-04-17)
Characterizing microbial communities via next-generation sequencing is subject to a number of pitfalls involving sample processing. The observed community composition can be a severe distortion of the quantities of bacteria actually present in the microbiome, hampering analysis and threatening the

Articles

DNA Standards for Metagenomic Research

DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.

DNA Standards for Metagenomic Research

DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.

DNA Standards for Metagenomic Research

DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.

DNA Standards for Metagenomic Research

DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.

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