M042080

Biotin Phosphoramidite

configured for PerkinElmer, configured for Polygen

• Synonyme(s) : Biotin amidite
• Formule empirique (notation de Hill): C₄₆H₆₄N₅O₈PS
• Numéro CAS: 1217500-22-5
• Poids moléculaire : 878.07
• Numéro MDL: MFCD00676535
• Code UNSPSC : 41116105
• ID de substance PubChem : 329817781
• Nomenclature NACRES : NA.21

Propriétés

• Gamme de produits: Proligo Reagents
• Pureté: ≥96% (³¹P-NMR)
• Adéquation: conforms to structure for H-NMR
• Compatibilité: configured for PerkinElmer; configured for Polygen
• Température de stockage: −20°C
• Chaîne SMILES: COc1ccc(cc1)C(N2C3CS[C@@H](CCCCC(=O)NCCOCCOCCOP(OCCC#N)N(C(C)C)C(C)C)C3NC2=O)(c4ccccc4)c5ccc(OC)cc5
• InChI: 1S/C46H64N5O8PS/c1-34(2)51(35(3)4)60(58-27-12-25-47)59-32-31-57-30-29-56-28-26-48-43(52)16-11-10-15-42-44-41(33-61-42)50(45(53)49-44)46(36-13-8-7-9-14-36,37-17-21-39(54-5)22-18-37)38-19-23-40(55-6)24-20-38/h7-9,13-14,17-24,34-35,41-42,44H,10-12,15-16,26-33H2,1-6H3,(H,48,52)(H,49,53)/t41-,42-,44-,60?/m0/s1
• Clé InChI: WSDQYLGSIUJJRU-ZMKGMYJVSA-N

Description

Description générale

Biotin phosphoramidite is a reagent used to directly incorporate a biotin label at the 5′ hydroxy group of the terminal nucleoside base through a p-aminophenethyl spacer group during oligonucleotide synthesis.

The photocleavable biotin phosphoramidite reagent is compatible with automated DNA/RNA synthesizers using phosphoramidite chemistry. The length of the p-aminophenethyl spacer group allows streptavidin affinity purification of the oligonucleotide by reverse-phase HPLC. Biotinlabeling is compatible with PCR and most hybridization techniques.Key Features of Biotin Phosphoramidite

• Behaves like any standard phosphoramidite on the DNA synthesizer
• Readily soluble in acetonitrile
• Biotin compound has a dimethoxytrityl (DMT) group on the ring structure,which allows coupling-yield determination and trityl-selective purification• A long, water-compatible linker arm ensures maximum sensitivity
• Provides a coupling efficiency of at least 95%The product is configured for Expedite^™ and PolyGen^® Synthesizers.

Application

Biotin Phosphoramidite is suitable for the synthesis of 5′-biotinylated oligonucleotides whose applications are seen in ligase-mediated gene detection, direct dideoxy sequencing following PCR, and non-radioactive sequencing of DNA.

Informations légales

Expedite is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

PolyGen is a registered trademark of PolyGen GmbH.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable