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L2643

Sigma-Aldrich

α-Lactose monohydrate

suitable for cell culture, BioReagent

Synonyme(s) :

β-D-Gal-(1→4)-α-D-Glc, 4-O-β-D-Galactopyranosyl-α-D-glucose, Milk sugar

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About This Item

Formule empirique (notation de Hill):
C12H22O11 · H2O
Numéro CAS:
5989-81-1
Poids moléculaire :
360.31
Numéro MDL:
MFCD00150747
Code UNSPSC :
12352201
ID de substance PubChem :
24896320
Nomenclature NACRES :
NA.75

Source biologique

bovine milk

Gamme de produits

BioReagent

Pureté

≥99% total lactose basis (GC)

Forme

powder

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤0.05% Glucose
≤4% β-lactose

Solubilité

water: 100 mg/mL, clear, colorless

Chaîne SMILES 

[H]O[H].OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C12H22O11.H2O/c13-1-3-5(15)6(16)9(19)12(22-3)23-10-4(2-14)21-11(20)8(18)7(10)17;/h3-20H,1-2H2;1H2/t3-,4-,5+,6+,7-,8-,9-,10-,11+,12+;/m1./s1

Clé InChI

WSVLPVUVIUVCRA-KPKNDVKVSA-N

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Application

α-Lactose monohydrate has been used:
  • as a component of fraction B of diluent M1 for dilution of ram semen
  • as a component of Platz diluent variant (PDV) for dilution of bottlenose dolphin semen
  • in the preparation of HyQ PF-Chinese hamster ovary (CHO) disaccharide media for the adaptation of CHO cell lines
  • for the preparation of protein-free chemically defined cell culture medium (PFCDM) for the adaptation of human embryonic kidney 293 (HEK293) cells

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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T R Robeck et al.
Biology of reproduction, 70(5), 1340-1348 (2004-01-09)
Research was conducted to develop an effective method for cryopreserving bottlenose dolphin (Tursiops truncatus) semen processed immediately after collection or after 24-h liquid storage. In each of two experiments, four ejaculates were collected from three males. In experiment 1, three
A Thiel et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 125, 528-539 (2019-02-06)
3-NOP (3-nitrooxypropanol) reduces enteric methane formation in ruminants. A series of ADME studies in rats, lactating goats and beef cattle was performed. 3-NOP was entirely absorbed from the GIT of rats: approximately 75% of the administered 3-NOP was eliminated as
L Anel et al.
Theriogenology, 60(7), 1293-1308 (2003-09-27)
Glycerol has been the most widely used cryopreservation agent for spermatozoa and a wide range of factors affect its action on sperm viability and fertilizing capacity. We tested three methods for freezing ram semen packed in 0.25 ml straws (final
Ling Zhang et al.
Theranostics, 10(24), 11339-11358 (2020-10-13)
Background: TSTA3 gene encodes an enzyme responsible for synthesis of GDP-L-fucose as the only donor in fucosylation. This study was designed to explore clinical value, function and underlying mechanism of TSTA3 in the development of esophageal squamous cell carcinoma (ESCC).
Dawn Sow Zong Leong et al.
Scientific reports, 7, 45216-45216 (2017-03-31)
Mammalian cells are generally considered to be unable to utilize polysaccharides for cell growth because the phospholipid bilayer in the cell membrane has very low permeability to sugars. With the recent discovery of the only known animal disaccharide transporter, a
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