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Key Documents

HPA002689

Sigma-Aldrich

Anti-WASHC1 antibody produced in rabbit

enhanced validation

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonyme(s) :

Anti-FAM39E, Anti-FLJ00038, Anti-WASH1

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About This Item

Code UNSPSC :
12352203
Numéro HPA (Human Protein Atlas):
Nomenclature NACRES :
NA.43

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Gamme de produits

Prestige Antibodies® Powered by Atlas Antibodies

Forme

buffered aqueous glycerol solution

Espèces réactives

human

Validation améliorée

RNAi knockdown
Learn more about Antibody Enhanced Validation

Technique(s)

immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:1000-1:2500

Séquence immunogène

GAPREVVDPSGGWATLLESIRQAGGIGKAKLRSMKERKLEKKKQKEQEQVRATSQGGHLMSDLFNKLVMRHKGISGKGPGAGEGPGGAFARVSDSIPPLPPPQQPQVDEDEDDWES

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

Description générale

WASH complex subunit 1 (WASHC1) is located on human chromosome 9p24.3. It belongs to Wiskott-Aldrich Syndrome Protein and SCAR Homolog (WASH) family. WASHC1 protein has an actin binding domain.

Immunogène

WASH complex subunit 1 recombinant protein epitope signature tag (PrEST)

Application

Anti-WASHC1 antibody produced in rabbit has been used:
  • in the detection of WASH complex in Dictyostelium cells by immunofluorescence imaging.
  • in the detection of WASH proteins in HeLa cells using confocal microscopy.
  • in western blotting for the detection of WASH1 complexes in human leukemic Jurkat T cells.

Actions biochimiques/physiologiques

WASH exists as multiple complex protein in HeLa and bovine cells. WASH proteins coordinate the migration of endosomal α5β1 integrin. WASHC1 protein regulates actin cytoskeleton organisation. WASH1 protein is regarded as actin-nucleating promoting factor. WASH protein complex interacts with actin related protein, Arp2/3 and mediates actin networking.

Caractéristiques et avantages

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Liaison

Corresponding Antigen APREST74318

Forme physique

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Informations légales

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

The Arp2/3 activator WASH regulates alpha5beta1-integrin-mediated invasive migration
Zech T, et al.
Journal of Cell Science, 124(22), 3753-3759 (2011)
Human subtelomeric WASH genes encode a new subclass of the WASP family
Linardopoulou EV, et al.
PLoS Genetics, 3(12), e237-e237 (2007)
Catherine M Buckley et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(40), E5906-E5915 (2016-09-21)
Macropinocytosis is an ancient mechanism that allows cells to harvest nutrients from extracellular media, which also allows immune cells to sample antigens from their surroundings. During macropinosome formation, bulk plasma membrane is internalized with all its integral proteins. It is
Functional characterization of Wiskott Aldrich Syndrome protein and scar homolog (WASH), a bi-modular nucleation promoting factor (NPF) able to interact with biogenesis of lysosome related organelle subunit 2 (BLOS-2) and gamma-tubulin
Monfregola J, et al.
The Journal of Biological Chemistry, jbc-M109 (2010)
WASH drives early recycling from macropinosomes and phagosomes to maintain surface phagocytic receptors
Buckley CM, et al.
Proceedings of the National Academy of Sciences of the USA, 113(40), E5906-E5915 (2016)

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