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DMN10

Sigma-Aldrich

GenElute™ Direct mRNA Miniprep Kits

sufficient for 10 purifications

Synonyme(s) :

GenElute™ Direct mRNA Miniprep Kit, GenElute™ mRNA Kit, Gen Elute

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About This Item

Code UNSPSC :
41105501
Nomenclature NACRES :
NA.52

Utilisation

sufficient for 10 purifications

Technique(s)

RNA purification: suitable

Température de stockage

15-25°C

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Description générale

The GenElute™ Direct mRNA Miniprep kit provides a convenient format to isolate polyadenylated mRNA directly from mammalian cells and tissues. The direct mRNA isolation procedure is based on that of Badley. Up to 107 mammalian cells or 40 mg tissue are lysed and homogenized, either with the filtration columns provided or with
a mechanical homogenizer. RNase is eliminated during a 10 minute proteinase K digestion. Sodium chloride is added, and polyadenylated RNA is captured on oligo(dT) polystyrene beads during a 10 minute incubation. For further enrichment, RNA may be released from the beads into fresh lysis solution and recaptured with the original beads. After 3 washes in a spin column, purified mRNA is eluted in 100 μl of 10 mM Tris-HCl, pH 7.4.

Application

The GenElute Direct Kit mRNA kit provides convenient procedures for isolating polyadenylated mRNA from previously prepared total RNA or directly from mammalian cells and tissues.

Caractéristiques et avantages

  • mRNA captured on oligo(dT) polystyrene beads in 10 minutes, with no mixing or rocking (Fig. 1)
  • Poly (A)+ mRNA isolated from total RNA in 40 minutes (Fig. 2) or 60 minutes directly from cells and tissues (Fig. 3)
  • Oligo(dT) polystyrene beads require fewer wash steps

Autres remarques

Use for isolating mRNA directly from mammalian cells or tissues.
For additional information, please see www.sigma-aldrich.com/mrna.

Informations légales

GenElute is a trademark of Sigma-Aldrich Co. LLC

Composants de kit seuls

Réf. du produit
Description
  • Elution solution 1.5 mL
  • Filtration columns with tubes 10 ea
  • Lysis solution 20 mL
  • 5 M NaCl 1.5 mL
  • Oligo(dT)-polystyrene beads .3 mL
  • Proteinase K 5 mg
  • 40% Glycerol solution .6 mL
  • Spin columns with tubes 10 ea
  • Collection tube 10 ea
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Pictogrammes

Health hazardCorrosionExclamation mark
GHS08,GHS05,GHS07

Mention d'avertissement

Danger

Mentions de danger

H290,H315,H319,H334,H335

Classification des risques

Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Brahmchetna Singh et al.
Molecular cancer, 6, 82-82 (2007-12-26)
Retinoids are potent growth inhibitory and differentiating agents in a variety of cancer cell types. We have shown that retinoids induce growth arrest in all pancreatic cancer cell lines studied, regardless of their p53 and differentiation status. However, the mechanism
Abhirami Visvanathan et al.
Genes, 10(2) (2019-02-20)
Despite recent advances in N⁶-methyladenosine (m⁶A) biology, the regulation of crucial RNA processing steps by the RNA methyltransferase-like 3 (METTL3) in glioma stem-like cells (GSCs) remains obscure. An integrated analysis of m⁶A-RIP (RNA immunoprecipitation) and total RNA-Seq of METTL3-silenced GSCs

Protocoles

Reverse Transcription Protocol

Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.

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