LoVo Cell Line human

87060101, human colon (supraclavicular lymph node metastasis), Epithelial

• Synonyme(s) : LoVo Cells
• Code UNSPSC : 41106514

Propriétés

• product name: LoVo Cell Line human, 87060101, from human colon(supraclavicular lymph node metastasis)
• Source biologique: human colon (supraclavicular lymph node metastasis)
• Mode de croissance: Adherent
• Caryotype: Modal no. 49, (2n=46)
• Morphologie: Epithelial
• Produits: Carcinoembryonic antigen (CEA)
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): metastasis
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human colon adenocarcinoma

Description de la lignée cellulaire

Derived from a metastatic tumour in the left supraclavicular region of a 56 year old male with adenocarcinoma of the colon. The cells produce carcino embryonic antigen (CEA).

Application

Human LoVo cell line is part of the three-dimensional (3D) heterotypic spheroid model developed to assess cancer immunotherapy agents in vitro. It has been used to test the in vitro cytotoxic property and anti-proliferative property of C. lechleri stem bark essential oil.

Tumourigenicity studies

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13,14
D13S317: 8,11
D16S539: 9,12
D5S818: 11,13
D7S820: 10,11
THO1: 9.3
TPOX: 8,9
vWA: 17,18

Milieu de culture

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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