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Key Documents

B8434

Sigma-Aldrich

Anti-BrdU antibody, Mouse monoclonal

clone BU-33, purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-BrdU antibody produced in mouse, Anti Brdu, Brdu Antibody, Brdu Antibody - Anti-BrdU antibody, Mouse monoclonal, Anti-Bromodeoxyuridine

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture
purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

BU-33, monoclonal

Forme

buffered aqueous solution

Espèces réactives

wide range

Conditionnement

antibody small pack of 25 μL

Concentration

~1.5 mg/mL

Technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using sections of intestine from mice or rats treated in vivo with BrdU

Isotype

IgG1

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

5′-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light. Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that is selectively incorporated into cell DNA at the S phase of the cell cycle.
Anti-BrdU antibody, Mouse monoclonal (mouse IgG1) is derived from the BU-33 hybridoma produced by the fusion of murine myeloma cells and splenocytes from BALB/c mouse immunized with bromodeoxyuridine (BrdU) conjugated to KLH.5′-Bromo-2-deoxyuridine (BrdU) staining is predominantly used to observe the multiplication of tumor cells and other tissues in vivo.

Spécificité

Reacts specifically with BrdU (bromodeoxyuridine) incorporated into DNA or coupled to a protein carrier. Cross-reaction with IdU (iododeoxyuridine) is observed.

Immunogène

bromodeoxyuridine conjugated to KLH.

Application

Anti-BrdU antibody has been used in:
  • BrdU labeling
  • immunohistochemistry
  • immunostaining
  • BrdU assay
Anti-BrdU antibody, Mouse monoclonal has been used in immunostaining, as a primary antibody:
  • to detect 5-fluorouridine-labelled RNA
  • to detect 5-bromo-2′-deoxyuridine (BrdU) labelled immunoreactive cells
  • against BrdU labelled rat retinal sections

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium axide.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilution samples should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Does 5-bromo-2'-deoxyuridine (BrdU) disrupt cell proliferation and neuronal maturation in the adult rat hippocampus in vivo?.
Hancock A, et al.
Behavioural Brain Research, 199(2), 218-221 (2009)
Controlling the number of melanopsin-containing retinal ganglion cells by early light exposure
Hong Jie, et al.
Experimental Eye Research, 17-26 (2013)
JAZ (Znf346), a SIRT1-interacting protein protects neurons by stimulating p21 (WAF/CIP1) expression.
Mallick S and D'Mello SR
The Journal of Biological Chemistry, jbc-M114 (2014)
PEG?PLA nanoparticles facilitate siRNA knockdown in adult zebrafish heart.
Diao J, et al.
Developmental Biology, 406(2), 196-202 null
Chronic treatment with the GLP1 analogue liraglutide increases cell proliferation and differentiation into neurons in an AD mouse model.
Parthsarathy V and Holscher C
PLoS ONE, 8(3), e58784-e58784 (2013)

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