B7263

N-tert-Butyl-α-phenylnitrone

≥98% (GC), cyclooxygenase-2 inhibitor, powder

• Synonyme(s) : N-Benzylidene-tert-butylamine N-oxide, PBN, Phenyl N-t-butylnitrone
• Formule linéaire : C₆H₅CH=N(O)C(CH₃)₃
• Numéro CAS: 3376-24-7
• Poids moléculaire : 177.24
• NACRES: NA.77
• PubChem Substance ID: 24891981
• UNSPSC Code: 12352202
• EC Number: 222-168-6
• MDL number: MFCD00008799
• Beilstein/REAXYS Number: 2044028

Propriétés

• Nom du produit: N-tert-Butyl-α-phenylnitrone, ≥98% (GC)
• Quality Segment: 100
• assay: ≥98% (GC)
• form: powder
• color: white
• mp: 73-74 °C (lit.)
• solubility: DMSO: soluble
• storage temp.: −20°C
• SMILES string: CC(C)(C)[N+](\[O-])=C\c1ccccc1
• InChI: 1S/C11H15NO/c1-11(2,3)12(13)9-10-7-5-4-6-8-10/h4-9H,1-3H3/b12-9-
• InChI key: IYSYLWYGCWTJSG-XFXZXTDPSA-N

Description

Application

N-tert-Butyl-α-phenylnitrone has been used:

• as a component of Dneasy Blood&Tissue buffer to preserve the oxidized state of DNA extracted from human non-tumorigenic epithelial breast (MCF10A) cells
• as a free radical scavenger of reactive oxygen species (ROS) in microglial (MG) cell lines
• to attenuate fibroblast senescence in unstable oral squamous cell carcinomas (GU-OSCC)

Biochem/physiol Actions

N-tert-butyl-α-phenylnitrone (PBN) is a commonly used free-radical spin trap.

N-tert-butyl-α-phenylnitrone (PBN) is a commonly used free-radical spin trap. It has been shown to reduce the number of emboli-induced cerebral microinfarctions in the rabbit cortex and prevent neoplasia by its radical scavenging activity and its ability to inhibit cyclooxygenase-2 activity. Reported to inhibit the induction of nitric oxide synthase (iNOS), thereby preventing the overproduction of nitric oxide (NO). PBN in a dose of 100 mg/kg i.p. reduced necrosis of the substantia nigra, pars reticulate in flurothyl-induced status epilepticus in rats. It protects against some types of post-trauma epileptogenic events in an animal model of epilepsy. The lethal dose of PBN in rats was found to be approximately 100 mg/100 g body weight (0.564 mmol/100Å g).

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)