A3682
Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Synonyme(s) :
Goat Anti-Mouse IgG (Fab specific)- HRP
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Produits recommandés
Source biologique
goat
Conjugué
peroxidase conjugate
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
secondary antibodies
Clone
polyclonal
Forme
buffered aqueous solution
Espèces réactives
mouse
Ne doit pas réagir avec
human, rat
Technique(s)
direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150
western blot: 1:80,000-160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per lane)
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
Description générale
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mice possess five classes of immunoglobulins- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat anti-mouse IgG (Fab specific)-peroxidase antibody is specific for mouse IgG and the Fab fragment of mouse IgG. The antibody reacts with all mouse IgG subclasses (G1, G2a, G2b and G3) and also with mouse IgA, IgM and IgE. Moreover, the antibody conjugate shows no reactivity with the Fc fragment of mouse IgG or with human and rat IgG.
Goat anti-mouse IgG (Fab specific)-peroxidase antibody is specific for mouse IgG and the Fab fragment of mouse IgG. The antibody reacts with all mouse IgG subclasses (G1, G2a, G2b and G3) and also with mouse IgA, IgM and IgE. Moreover, the antibody conjugate shows no reactivity with the Fc fragment of mouse IgG or with human and rat IgG.
Immunogène
purified mouse IgG, Fab fragment
Application
Murine IgG levels were quantitated in brain abscess homogenates by ELISA using HRP conjugated goat anti-mouse Fab specific antibody as the detection antibody.
Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in goat has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemical studies
- dot or immunoblotting
Goat anti-mouse IgG (Fab specific)-peroxidase antibody can be used for western blotting applications. The product can also be used for direct ELISA (1:40,000) and immunohistochemistry (at 1:150, using formalin-fixed, paraffin-embedded sections).
HRP conjugated goat anti-mouse IgG Fab specific was used as the secondary antibody for western blot analysis of bacterial samples of s. equi cells to detect fibrinogen-binding proteins.
Actions biochimiques/physiologiques
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG2a has cytotropic properties and IgG2 molecules have the ability to activate the complement system. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Forme physique
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT
Notes préparatoires
Adsorbed with human IgG and rat serum proteins to reduce background staining with human or rat samples.
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Astrocytes produce the antiinflammatory and neuroprotective agent hydrogen sulfide
Lee M, et al.
Neurobiology of Aging, 30(10), 1523-1534 (2009)
Dirk Pohlers et al.
Arthritis research & therapy, 9(3), R59-R59 (2007-06-28)
Genome-wide gene expression was comparatively investigated in early-passage rheumatoid arthritis (RA) and osteoarthritis (OA) synovial fibroblasts (SFBs; n = 6 each) using oligonucleotide microarrays; mRNA/protein data were validated by quantitative PCR (qPCR) and western blotting and immunohistochemistry, respectively. Gene set
M Meehan et al.
Microbiology (Reading, England), 147(Pt 12), 3311-3322 (2001-12-12)
The major cell-wall-associated protein of the equine pathogen Streptococcus equi subsp. equi is an M-like fibrinogen-binding protein (FgBP) which binds equine fibrinogen (Fg) avidly, through residues located at the extreme N-terminus of the molecule. In this study, it is shown
Moonhee Lee et al.
Journal of neuroinflammation, 13, 32-32 (2016-02-10)
Sodium thiosulfate (STS) is an industrial chemical which has also been approved for the treatment of certain rare medical conditions. These include cyanide poisoning and calciphylaxis in hemodialysis patients with end-stage kidney disease. Here, we investigated the anti-inflammatory activity of
Acidic fibroblast growth factor (FGF) potentiates glial-mediated neurotoxicity by activating FGFR2 IIIb protein
Lee M, et al.
The Journal of Biological Chemistry, 286(48), 41230-41245 (2011)
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique