09978

Sulfate d'ammonium

BioUltra, ≥99.0% (T)

• Synonyme(s) : Mascagnite
• Formule linéaire : (NH₄)₂SO₄
• Numéro CAS: 7783-20-2
• Poids moléculaire : 132.14
• Numéro CE : 231-984-1
• Numéro MDL: MFCD00003391
• Code UNSPSC : 12352314
• eCl@ss : 38030408
• ID de substance PubChem : 329748870
• Nomenclature NACRES : NA.26

Propriétés

• Gamme de produits: BioUltra
• Pureté: ≥99.0% (T)
• Forme: crystals
• Impuretés: insoluble matter, passes filter test
• Résidus de calcination: ≤0.01% (as SO₄)
• Perte: ≤0.1% loss on drying, 110 °C
• pH: 5.0-6.0 (25 °C, 1 M in H₂O)
• Solubilité: H₂O: 1 M at 20 °C, clear, colorless
• Traces d'anions: chloride (Cl^-): ≤5 mg/kg; nitrate (NO₃^-): ≤50 mg/kg; phosphate (PO₄^3-): ≤5 mg/kg
• Traces de cations: Al: ≤5 mg/kg; As: ≤0.2 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤1 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤2 mg/kg; Fe: ≤2 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤50 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤2 mg/kg; Sr: ≤5 mg/kg; Zn: ≤1 mg/kg
• λ: 1 M in H₂O
• Absorption UV: λ: 260 nm A_max: ≤0.037; λ: 280 nm A_max: ≤0.030
• Chaîne SMILES: N.N.OS(O)(=O)=O
• InChI: 1S/2H3N.H2O4S/c;;1-5(2,3)4/h2*1H3;(H2,1,2,3,4)
• Clé InChI: BFNBIHQBYMNNAN-UHFFFAOYSA-N

Description

Description générale

Ammonium sulfate is naturally present as a rare mineral mascagnite in volcanic fumaroles and due to coal fires in some dumps.

Application

Ammonium sulfate has been used:

• to prepare mobile phase A for hydrophobic interaction chromatography (HIC)
• as reference material for the thermal decomposition of NH₄NO₃ (S1–S6) thermal decomposition to N₂O (S1-N₂O–S6-N₂O)
• to prepare the stock solution for M63 medium
• as a reagent in molecular biology and chromatography
• in the precipitation and fractionation of proteins
• in the purification of antibodies
• for the crystallographic analysis of nucleic acids and proteins
• in high-performance liquid chromatography (HPLC) analysis of proteins, such as in hydrophobic interaction chromatography
• for Sepharose chromatography of tRNAs using reverse salt gradients
• in long PCR buffer, in PCR lysis solution, and second-strand cDNA synthesis
• HPLC analysis of phospholipids

Actions biochimiques/physiologiques

Ammonium sulfate is an inorganic salt, which is mostly used as a soil fertilizer. It is also used as a flame retardant since it elevates the combustion temperature of the material and lowers maximum weight loss rates. This leads to an upsurge in the production of residue or char.

Autres remarques

The most commonly used salt for the fractional precipitation of proteins due to its solubility and effectiveness; Protein crystallization; Sepharose^™ chromatography of tRNAs using reverse salt gradients.

Informations légales

Sepharose is a trademark of Cytiva

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)