EZHS100B

Human S100B ELISA Kit

quantifies S100B in 50 μL cerebrospinal fluid, serum or plasma

• Synonyme(s) : Protein S100B, S-100 protein beta chain, S100 calcium-binding protein B
• Code UNSPSC : 12161503
• eCl@ss : 32161000
• Nomenclature NACRES : NA.84

Propriétés

• product name: Human S100B ELISA, EZHS100B-33K, This Human S100B ELISA kit is to be used for the quantification of human S100B in cerebrospinal fluid, serum & plasma.
• Niveau de qualité: 200
• Espèces réactives: human
• Conditionnement: kit of 1 × 96 wells
• Paramètres: 50 μL sample volume (4hr assay)
• assay range: accuracy: 93%; (CSF); accuracy: 94%; (serum); sensitivity: 2.7 pg/mL; standard curve range: 2.7-2000 pg/mL; inter-assay cv: <4%; intra-assay cv: <4%
• Technique(s): ELISA: suitable
• Entrée: sample type cerebrospinal fluid (CSF); sample type plasma (K2 EDTA); sample type serum
• Numéro d'accès UniProt: P04271
• Application(s): research use
• Méthode de détection: colorimetric (450nm)
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

EMD Millipore’s Human S100B ELISA kit is to be used for the quantification of human S100B in cerebrospinal fluid, serum and plasma. This kit has not been tested for cell culture supernatants.

S100B is a calcium-binding protein produced mainly by astrocytes in the central nervous system and has been implicated in the development and maintenance of the nervous system. Although the mechanism of S100B secretion is unknown, it appears to be affected by oxidative stress. At nanomolar concentrations (normal levels), S100B is able to protect neurons against glutamate toxicity. Glial damage or astrocytic reactions to neural injury (reactive astrogliosis) causes an increase in S100B to micromolar concentrations. High levels of S100B will further increase neuronal damage. High levels of S100B are considered a biomarker that could indicate damage or dysfunction of CNS.

Spécificité

The antibody pair used in this assay measures Human S100B and has no cross-reactivity with S100A1, S100A6 and S100A13.

This assay detects S100B in multiple species, including: bovine, hamster, horse, monkey, mouse, pig, rabbit, and rat.

Application

Research Category
Neuroscience

This is a same day assay

Used to detect/quantify: S100B

Autres remarques

Please contact Technical Service for linearity of dilution.

Clause de non-responsabilité

For research use only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Pictogrammes: GHS06,GHS05,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H290,H302 + H332,H311,H317,H411
• Conseils de prudence: P234 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312
• Classification des risques: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects