ABT273

Anti-SUN1 Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : SUN domain-containing protein 1, Protein unc-84 homolog A, Sad1/unc-84 protein-like 1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: rat, human
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_001124437
• Numéro d'accès UniProt: O94901
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SUN1(23353)

Description

Description générale

SUN domain-containing protein 1 (SUN1), also known as Protein unc-84 homolog A or Sad1/unc-84 protein like 1 (UNC84A), is a component of the SUN-protein-containing complexes also called LINC (linker of nucleoskeleton and cytoskeleton) complexes. These complexes link the nucleoskeleton and cytoskeleton structures together in a scaffold like fashion. SUN1 is required for interkinetic nuclear migration and nucleokinesis as well as nucleus-centrosome coupling during radial neuronal migration and glial migration in the cortex. SUN1 also is important during meiosis and is required for proper gametogenesis as well. Additionally, SUN1 it is important in the distribution of nuclear pore complexes on the nuclear membrane. Interestingly, LINC complexes are also critical for vital functions such as hearing. For instance, recent research demonstrates that the position of the nucleus in sensory epithelial cells, partly governed by SUN1, appears critical for normal hearing.

Immunogène

Epitope: Perinuclear space domain

MBP-tagged recombinant protein corresponding to the perinuclear space domain of human SUN1.

Application

Detect SUN1 using this Anti-SUN1 antibody validated for use in western blotting & ICC.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Western Blotting Analysis: A 1:1;000 dilution from a representative lot detected SUN1 in 10 µg of HepG2, Jurkat, and PC3 cell lysate.
Western Blotting Analysis: A representative lot detected SUN1 recombinant protein (Haque, F., et al. (2010). Journal of Biol. Chem. 285(5): 3487-3498).
Immunocytochemistry Analysis: A representative lot detected SUN1 in human foreskin fibroblasts cells (Haque, F., et al. (2010). Journal of Biol. Chem. 285(5): 3487-3498).

Qualité

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1;000 dilution of this antibody detected SUN1 in 10 µg of A431 cell lysate.

Description de la cible

~ 110 kDa observed. The calculated molecular weight is 90 kDa, however Sun1 has been shown as a ~80-110 kDa band in western blots (Gob, E., et al. (2011). Commun. Integr Biol. 4(4):440-442). Uncharacterized band(s) may appear in some lysates.

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable