ABC945

Anti-GMPR Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : GMP reductase 1, Guanosine 5′-monophosphate oxidoreductase 1, Guanosine monophosphate reductase 1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): primate (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)
• Technique(s): immunohistochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_006868
• Numéro d'accès UniProt: P36959
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... GMPR(2766)

Description

Description générale

GMP reductase 1 (EC 1.7.1.7; UniProt P36959; also known as Guanosine 5′-monophosphate oxidoreductase 1, Guanosine monophosphate reductase 1) is encoded by the GMPR (also known as GMPR1) gene (Gene ID 2766) in human. De novo biosynthesis of Guanosine 5′-monophosphate (GMP) is accomplished by reactions catalyzed by several enzymes. Inositol monophosphate (IMP) dehydrogenase 1 and 2 (IMPDH1 and IMPDH2) convert IMP into xanthosine monophosphate (XMP), GMP synthetase (GMPS) then converts XMP into GMP, while guanosine monophosphate reductase (GMPR) catalyzes the reversible conversion of GMP to IMP to fuel back into both the AMP and GMP synthesis pathways. GMPR is shown to reduce intracellular GTP pools, thereby downregulating the level of several GTP-bound (active) RHO-GTPases that are essential for invadopodia formation, extracellular matrix degradation and invasion of melanoma cells both in cultures in vitro and in tumor xenografts in vivo. Consistent with its role as a negative regulator of the invasive melanoma phenotype, GMPR expression is reported to be either undetectable or drastically reduced in human melanoma cell lines when compared to normal melanocytes.

Spécificité

This polyclonal antibody detects human GMP reductase 1 (GMPR) and stained predominantly the cytoplasm of cells in human tissue sections by immunohistochemistry.

Immunogène

Epitope: Between the second and third GMP-binding region.

KLH-conjugated linear peptide corresponding a sequence between the second and third GMP-binding region of human GMPR.

Application

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected GMPR in human prostate cancer, as well as in normal testis and skeletal muscle tissue sections.

Research Category
Apoptosis & Cancer

Research Sub Category
Cytoskeletal Signaling

This Anti-GMPR Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of GMPR.

Qualité

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected GMPR in 10 µg of human skeletal muscle tissue lysate.

Description de la cible

~35 kDa observed. 37.42 kDa calculated.

Forme physique

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1