AB5638P

Anti-Cannabinoid Receptor 1 Antibody, CT

Chemicon^®, from rabbit

• Synonyme(s) : CB1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: rat, mouse
• Fabricant/nom de marque: Chemicon^®
• Technique(s): ELISA: suitable; western blot: suitable
• Numéro d'accès NCBI: NM_016083.3; NM_033181.2
• Numéro d'accès UniProt: P21554
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Cnr1(12801)

Description

Description générale

CB1 is a protein that is one of two cannabinoid receptors (CB1 CB2). Cannabinoid principally delta-9-tetrahydrocannabinol (THC) and its synthetic analogs, are the active psychoactive compounds present is marijuana. These cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptors which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Three splice variants have been found for this gene. CB1 is primarily located in brain, and CB2 is found macrophages.

Spécificité

Recognizes rat Cannabinoid Receptor 1 (CB1). The immunogen shows no significant sequence homology with CB2 or other receptors.

SPECIES REACTIVITIES: The immunogen peptide is 100% conserved mouse, 94% in monkey and human, 88% in zebra finch, 75% in feline and newt, (salamander) and 73% in puffer fish. Reactivity with other species has not been confirmed.

Immunogène

A 20 amino acid peptide sequence near the cytoplasmic, C-terminus of rat CB1 (Gerard et al. 1991; Gerard et al.

1990; Shire et al. 1995; Matsuda et al. 1990; Ho et al. 1996; Chakraborty et al. 1995).

Epitope: C-terminus

Application

Anti-Cannabinoid Receptor 1 Antibody, C-terminus is an antibody against Cannabinoid Receptor 1 for use in ELISA & WB.

Research Category
Neuroscience

Research Sub Category
Neuroinflammation & Pain

Neurotransmitters & Receptors

Western blot: 1-10 μg/mL using ECL. Expected sizes are ~60kDa and 80-84kDa under reducing conditions.

Immunohistochemistry: not tested. It is recommended that the antibody be tried at 2-10 μg/mL on paraformaldehyde fixed tissue.

ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide (Catalog number AG213) per well.

Optimal working dilutions must be determined by the end user.

Forme physique

Affinity purified immunoglobulin. Liquid in phosphate buffered saline containing 0.1% BSA.

Stockage et stabilité

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable