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475821

Sigma-Aldrich

Microcystin-LR

from Microcystis aeruginosa, ≥95% (HPLC), liquid, protein phosphatase 2A and protein phosphatase 1 inhibitor, Calbiochem®

Synonyme(s) :

InSolution Microcystin-LR, Microcystis aeruginosa

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About This Item

Formule empirique (notation de Hill):
C49H74N10O12
Poids moléculaire :
995.17
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77

product name

Microcystin-LR, Microcystis aeruginosa, InSolution, ≥95%, inhibitor of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A)

Niveau de qualité

100

Pureté

≥95% (HPLC)

Forme

liquid

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
desiccated (hygroscopic)
protect from light

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

Cyclic heptapeptide toxin isolated from the freshwater cyanobacteria, Microcystis aeruginosa. Potent inhibitor of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A). Unlike Okadaic Acid (Cat. No. 495604), microcystin-LR is effective on both PP1 (IC50 = 1.7 nM) and PP2A (IC50 = 40 pM). Reacts with phosphatase in a two-step mechanism involving rapid binding and inactivation of the catalytic subunit followed by slower covalent interactions. Has no effect on protein kinases, making it useful for reducing contaminating phosphatase activities in protein kinase assays.

Actions biochimiques/physiologiques

Cell permeable: no
Primary Target
protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A)
Product does not compete with ATP.
Reversible: no
Target IC50: 1.7 nM, 40 pM, against protein phosphatase 1 (PP1), protein phosphatase 2A (PP2A), respectively

Conditionnement

Packaged under inert gas
yes

Avertissement

Toxicity: Highly Toxic (H)

Forme physique

A 2.5 mM (250 µg/100 µl) solution of Microcystin-LR, Microcystis aeruginosa (Cat. No. 475815) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Autres remarques

Craig, M., et al. 1996. Biochem. Cell Biol. 74, 569.
Nishiwaki, R., et al. 1994. Cancer Lett. 83, 283.
Amick, G.D., et al. 1992. Biochem. J.287, 1019.
Davidson, H.W., et al. 1992. J. Cell Biol.116, 1343.
Nishiwaki-Matsushima, R., et al. 1992. J. Cancer Res. Clin. Oncol. 118, 420.
Cohen, P., et al. 1990. Trends Biochem. Sci.15, 98.
MacKintosh, C., et al. 1990. FEBS Lett.264, 187.
Cohen, P., et al. 1989. FEBS Lett.250, 596.
Carmichael, W.W. 1988. in Handbook of Natural Toxins3,Marine Toxins and Venoms (Tu, A.T., Ed.) p. 121, Decker, New York.
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Informations légales

Not available for sale outside of the United States.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

188.6 °F - closed cup - (Dimethylsulfoxide)

Point d'éclair (°C)

87 °C - closed cup - (Dimethylsulfoxide)

Certificats d'analyse (COA)

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