208731

Calpain Substrate II, Fluorogenic

Cyclic fluorogenic substrate for the quantitative determination of calpain -1 and -2.

• Synonyme(s) : Calpain Substrate II, Fluorogenic, Suc-LY-AMC
• Formule empirique (notation de Hill): C₂₉H₃₁N₃O₇
• Numéro CAS: 94367-20-1
• Poids moléculaire : 533.57
• Numéro MDL: MFCD00057900
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 100
• Pureté: ≥95% (TLC)
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated; protect from light
• Couleur: off-white
• Solubilité: DMSO: soluble
• Fluorescence: λ_ex ~380 nm; λ_em ~460 nm
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• InChI: 1S/C29H33N3O8/c1-4-16(2)27(32-24(34)11-12-25(35)36)29(39)31-22(14-18-5-8-20(33)9-6-18)28(38)30-19-7-10-21-17(3)13-26(37)40-23(21)15-19/h5-10,13,15-16,22,27,33H,4,11-12,14H2,1-3H3,(H,30,38)(H,31,39)(H,32,34)(H,35,36)/t16?,22?,27-/m0/s1
• Clé InChI: VSWNAJAOJZXWBM-FTJGSZSVSA-N

Description

Description générale

Cyclic fluorogenic substrate for the quantitative determination of calpain -1 and -2. Also suitable for measuring the peptidase activity of the 20S proteasome. Typical concentrations range from 10 - 100 µM, depending on assay conditions.

Fluorogenic substrate for the quantitative determination of porcine calpain I and II activity. Also serves as a substrate for other proteases including proteasome. (excitation wavelength: 380 nm; emission wavelength: 460 nm)

Actions biochimiques/physiologiques

Cell permeable: no

Primary Target
Calpain -1 and -2

Product does not compete with ATP.

Reversible: no

Avertissement

Toxicity: Standard Handling (A)

Séquence

Suc-Leu-Tyr-AMC

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Autres remarques

Seol, J.H., et al. 1989. FEBS Lett. 247, 197.
Woo, K.M., et al. 1989. J. Biol. Chem. 264, 2088.
Sasaki, T., et al. 1984. J. Biol. Chem.259, 12489.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable