14-326-M
Src Protein, active, 10 µg
Active, N-Terminal His6-tagged, recombinant, full-length, human Src. For use in Kinase Assays.
Synonyme(s) :
SRC1
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About This Item
Code UNSPSC :
12352202
eCl@ss :
32160405
Nomenclature NACRES :
NA.26
Produits recommandés
Source biologique
human
Niveau de qualité
Produit recombinant
expressed in Sf21 cells
Forme
liquid
Poids mol.
Mw 61.7 kDa
Fabricant/nom de marque
Upstate®
Technique(s)
activity assay: suitable (kinase)
Solubilité
soluble
Numéro d'accès NCBI
Numéro d'accès UniProt
Informations sur le gène
human ... SRC(6714)
Description générale
Research area: Cell Signaling
N-Terminal His6-tagged, recombinant, full-length, human Src.
Src proteins are non-receptor protein tyrosine kinases, that belong to the Src family of kinases. These proteins have a unique structure consisting of an N-terminal region with a 14-carbon myristoyl group, two Src homology domains (SH2 and SH3), a catalytic tyrosine-protein kinase domain (SH1), and a short C-terminal tail. The SH1 domain is catalytic in function, while the SH2 and SH3 domains are non-catalytic and regulatory in nature.
Product Source: Human c-src, expressed in Sf21 cells.
N-Terminal His6-tagged, recombinant, full-length, human Src.
Src proteins are non-receptor protein tyrosine kinases, that belong to the Src family of kinases. These proteins have a unique structure consisting of an N-terminal region with a 14-carbon myristoyl group, two Src homology domains (SH2 and SH3), a catalytic tyrosine-protein kinase domain (SH1), and a short C-terminal tail. The SH1 domain is catalytic in function, while the SH2 and SH3 domains are non-catalytic and regulatory in nature.
Product Source: Human c-src, expressed in Sf21 cells.
Application
The Src protein may be used to study the interaction between CD133 and Src protein and its effect on FAK phosphorylation and cell migration through in vitro kinase assay.
Actions biochimiques/physiologiques
Src protein tyrosine kinases are involved in signaling pathways that control a plethora of cellular functions ranging from cell cycle, proliferation, differentiation, gene transcription, immune responses, apoptosis, etc. Upon its activation, Src phosphorylates and activates several other kinases Like MAPK (Mitogen-Activated Protein Kinase), p38, and ERK (Extracellular signal-regulated kinase). Activated MAPK (Mitogen-Activated Protein Kinase) regulates inflammation, cell development, differentiation and senescence. P38 is involved with the migration and survival of endothelial cells and extracellular signal-regulated kinase (ERK) promotes the proliferation and inflammation of endothelial cells. They play a key role in the regulation of cytoskeletal organization through the phosphorylation of substrates like Actin filament-associated protein 1 (AFAP1) and cortactin (CTTN). Src also plays a crucial role in maintaining cell adhesion, morphology, motility, and bone resorption by interacting with focal adhesion kinases. Src tyrosine kinase activity is upregulated in a variety of cancers including colon, lung, and pancreatic cancer, etc.
Conditionnement
2 vials at 5μg each
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-326M when ordering the 250μg size.
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-326M when ordering the 250μg size.
Qualité
Routinely evaluated by phosphorylation of Src Substrate Peptide
Autres remarques
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Protein tyrosine kinases, with emphasis on the Src family.
Courtneidge, S A
Seminars in Cancer Biology, 5, 239-246 (1994)
Jianhui Ma et al.
Cancer cell, 35(3), 504-518 (2019-03-05)
Ionizing radiation (IR) and chemotherapy are standard-of-care treatments for glioblastoma (GBM) patients and both result in DNA damage, however, the clinical efficacy is limited due to therapeutic resistance. We identified a mechanism of such resistance mediated by phosphorylation of PTEN
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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