05-1064

Anti-Phospho-Stat1 (Tyr701) Antibody, clone 5C9.2

clone 5C9.2, from mouse

• Synonyme(s) : Transcription factor ISGF-3 components p91/p84, signal transducer and activator of transcription 1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 5C9.2, monoclonal
• Espèces réactives: rat, mouse, human
• Technique(s): western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_009330
• Numéro d'accès UniProt: P42224
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: phosphorylation (pTyr701)
• Informations sur le gène: human ... STAT1(6772) ; mouse ... Stat1(20846)

Description

Description générale

STATs (Signal-Transducing Activators of Transcription) are the principle substrates of JAK kinases, and mediators of cytokine signaling. STAT proteins have SH2 domains, and once phosphorylated by JAK kinases, STATs dimerize in a head-to-tail fashion, through their SH2 domains. STAT1 is a critical mediator of IFN signaling, STAT4 is specific to IL-12, STAT6 is activated primarily by IL-4 and IL-13, while STAT5 A and B appear to be most important to the function of growth hormone and prolactin. STAT3 is involved in multiple signaling pathways, and mice deficient in it are not viable. In addition to tyrosine phosphorylation, many STAT proteins are targets of Ser/Thr kinases, and those phosphorylation events potentiate STAT-induced transcription. The phosphorylation state of STATs can be monitored by phosphorylation state-specific antibodies.

Spécificité

This antibody recognizes phosphorylated STAT1 at tyrosine 701.

Immunogène

Epitope: Phosphorylated Tyr701

KLH-conjugated linear peptide corresponding to the region around phosphorylated tyrosine 701.

Application

Anti-Phospho-Stat1 (Tyr701) Antibody, clone 5C9.2 detects level of Phospho-Stat1 (Tyr701) & has been published & validated for use in WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Qualité

Routinely evaluated by western blot on EGF treated and untreated A431 cell lysate.

Western Blot Analysis: 0.5-2 µg/mL of this lot was used to detect STAT1 phosphorylated at Tyr701 in EGF treated and untreated A431 cell lysates.

Description de la cible

88-92 kDa

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS and 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8ºC from date of receipt.

Remarque sur l'analyse

Control
EGF treated and untreated A431 cell lysates, PDGF treated and untreated NIH/3T3 cell lysates, pervanadate treated and untreated PC12 cell lysates.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable