803219

Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))

• Synonyme(s) : Ethylene glycolbis(sulfosuccinimidylsuccinate), bis(sulfosuccinimidyl) 4,4′-[(ethylene)dioxy]bis(4-oxobutanoate)
• Formule empirique (notation de Hill): C₁₈H₁₈O₁₈N₂Na₂S₂
• Numéro CAS: 167410-92-6
• Poids moléculaire : 660.45
• Numéro MDL: MFCD00054988
• Code UNSPSC : 12352200
• ID de substance PubChem : 329768789
• Nomenclature NACRES : NA.22

Propriétés

• Forme: powder
• Niveau de qualité: 100
• Poids mol.: 660.45
• Pertinence de la réaction: reagent type: cross-linking reagent
• Conditions de stockage: desiccated
• Solubilité: water: soluble
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• InChI: 1S/C18H20N2O18S2.2Na/c21-11-7-9(39(29,30)31)17(27)19(11)37-15(25)3-1-13(23)35-5-6-36-14(24)2-4-16(26)38-20-12(22)8-10(18(20)28)40(32,33)34;;/h9-10H,1-8H2,(H,29,30,31)(H,32,33,34);;/q;2*+1/p-2
• Clé InChI: IYBKWXQWKPSYDT-UHFFFAOYSA-L

Description

Description générale

EGS is a water-insoluble, homobifunctional N-hydroxysuccinimide ester (NHS ester) and Sulfo-EGS is its water-soluble analog. The spacer arm contains two cleavable ester sites that may be broken with hydroxylamine, which yields two fragments with terminal amide bonds and the release of ethylene glycol. These reagents are often used for conjugating radiolabeled ligands to cell surface receptors. The water insoluble reagent EGS does not possess a charged group, is lipophilic and, therefore, membrane-permeable and useful for intracellular and intramembrane protein conjugation. EGS is first dissolved in DMSO or DMF and added to the aqueous reaction mixture at a final solvent concentration of 10-20%, to minimize detrimental affects to the protein.

Caractéristiques et avantages

• Reactive groups: sulfo-NHS ester (both ends)
• Reactive towards: amino groups (primary amines)
• Water-soluble; compare to EGS
• Membrane-impermeable, allowing for cell surface labeling
• Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
• Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS

Attention

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H315,H319,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable