SAB4200788

Anti-Human IgG1 (Fc specific) antibody, Mouse monoclonal

clone HP-6001, purified from hybridoma cell culture

• Synonym(s): Anti-Human immunoglobulin G1
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: HP-6001, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): indirect ELISA: 0.5 μg/mL using 1 μg/mL Human IgG1 myeloma protein for coating.
• isotype: IgG2b
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Human IgGs consist of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG, respectively. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens and pathological conditions. For instance, anti-polysaccharide responses are mainly of the IgG2 subclass while protein antigens give rise to IgG1 and IgG3 antibodies. Human IgG1 is the predominant subclass of in vivo and in vitro produced anti-tetanus toxoid antibodies. IgG1 and IgG3 are the only subclasses capable of adherence to mononuclear phagocytes and are recognized readily by the Fc receptors on various reticulo-endothelial cells while IgG2 and IgG4 are far less efficient.

The amount of the different IgG subclasses present in the bloodstream varies with age. For example, IgG1 and IgG3 reach normal adult levels by 5-7 years of age while IgG2 and IgG4 levels raise more slowly, reaching adult levels at about 10 years of age. Serum IgG subclass deficiencies have been recorded for different patient groups. For example, a disproportionate elevation of IgG1 has been found in the cerebral spinal fluid of patients with multiple sclerosis.Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and may assist in the diagnosis of various disorders.

Monoclonal Anti-Human IgG1 (Fc specific) specifically recognizes Fc region of Human IgG1. The antibody shows no cross-reactivity with human IgG2, IgG3 and IgG4. This clone has been established as a useful human IgG1 specificity standard by the WHO/IUIS.

Immunogen

Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads

Application

The antibody is recommended to use in various immunological techniques, including ELISA.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable