R406-05A

Rat Osteoblasts: Rob, adult

• UNSPSC Code: 41106514
• NACRES: NA.81

Properties

• biological source: rat bone (Rat bone)
• Quality Level: 100
• packaging: pkg of 500,000 cells
• manufacturer/tradename: Cell Applications, Inc
• growth mode: Adherent
• morphology: osteoblast
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): arthritis; osteoporosis
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

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ROb from Cell Applications, Inc. have been used to evaluate a novel hydroxyapatite (HAp)/poly(l-lactic acid) (PLLA) nanocomposite nonwoven fabric, coated with calcined HAp nanocrystals as a material for bone regeneration and demonstrated better spreading on the nanocrystal-coated vs. uncoated surface (Yanagida, 2011). Characterization: Positive for bone mineralization.

Cell Line Origin

Bone

Application

Model for skeletal system research, signaling pathways, bone morphogenesis, gene expression, cell activation and proliferation, wound healing, bone formation and regeneration, treatment strategies, tissue engineering, in vitro bone models, mineralization, hormone treatments.

Components

Rat Osteoblast Basal Medium that contains 10% FBS and 10% DMSO

Quality

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

Preparation Note

• 1st passage, >500,000 cells in Rat Osteoblast Basal Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 10 population doublings

Subculture Routine

Please refer to the ROb Culture Protocol.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable