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05-840

Sigma-Aldrich

Anti-IDO (Indoleamine 2,3-Dioxygenase) Antibody, clone 10.1

clone 10.1, Upstate®, from mouse

Synonym(s):

Indoleamine 2,3-dioxygenase, Indoleamine-pyrrole 2,3-dioxygenase, indole 2,3-dioxygenase, indoleamine-pyrrole 2,3 dioxygenase, IDO1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

10.1, monoclonal

species reactivity

human, mouse

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... IDO1(3620)
mouse ... Ido1(15930)

General description

Indoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO may be involved in the suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.

Specificity

Recognizes IDO (Indoleamine 2,3- Dioxygenase), Mr 42 kDa.

Immunogen

GST fusion protein corresponding to residues 78-184 of human Indoleamine 2,3- Dioxygenase (IDO). Clone 10.1.

Application

Anti-IDO (Indoleamine 2, 3-Dioxygenase) Antibody, clone 10.1 is an antibody against IDO (Indoleamine 2 for use in IC & WB.
Immunocytochemistry: A previous lot was used by an independent laboratory in Phoenix cells transfected with an expression vector.

Immunohistochemistry: A previous lot of this antibody was used to detect IDO in human retina, primary eye tumor and metastatic liver tumor tissue sections (Chen, P.W., et al. Experimental Eye Research 85(2007) 617-625).

Quality

Routinely evaluated by Western Blot on untreated and IFNgamma treated (24 hrs) Hela lysates.

Western Blot Analysis:
1:500 dilution of this lot detected IDO on 10 ug of untreated and IFN gamma treated Hela lysates.

Target description

42 kDa

Linkage

Replaces: 04-1056

Physical form

Format: Purified
Purified mouse monoclonal IgG in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.

Analysis Note

Control
Human IFN-gamma stimulated HeLa cell lysates, IFN gamma stimulated human peripheral blood lymphocytes.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Annabel Meireson et al.
Frontiers in immunology, 12, 736498-736498 (2021-09-25)
Immune escape is an early phenomenon in cancer development/progression. Indoleamine 2,3-dioxygenase 1 (IDO1) is a normal endogenous mechanism of acquired peripheral immune tolerance and may therefore be tumor-promoting. This study investigated the clinical relevance of IDO1 expression by immune cells
Mechanism of interferon-gamma action. Characterization of indoleamine 2,3-dioxygenase in cultured human cells induced by interferon-gamma and evaluation of the enzyme-mediated tryptophan degradation in its anticellular activity.
Takikawa, O, et al.
The Journal of Biological Chemistry, 263, 2041-2048 (1988)
Ying Lu et al.
Blood, 119(4), 1075-1085 (2011-12-02)
Allogeneic hematopoietic stem cell transplantation (HSCT) can eradicate chemorefractory leukemia through the graft-versus-leukemia (GVL) activity of donor T cells. However, the clinical success of allo-HSCT is limited by the graft-versus-host disease (GVHD) activity of donor T cells. We have reported
Tryptophan and the immune response.
Moffett, John R and Namboodiri, Ma Aryan
Immunology and Cell Biology, 81, 247-265 (2003)
Uveal melanoma expression of indoleamine 2,3-deoxygenase: establishment of an immune privileged environment by tryptophan depletion.
Peter W Chen,Jessamee K Mellon,Elizabeth Mayhew,Shixuan Wang,Yu Guang He,Nick Hogan et al.
Experimental Eye Research null

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