HPLC Analysis of Sibutramine Enantiomers on Astec® CHIROBIOTIC® V
CONDITIONS
column
CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)
mobile phase
15 mM ammonium formate in methanol
flow rate
1 mL/min
column temp.
25 °C
detector
UV, 230 nm
injection
5 μL
sample
1 mg/mL in methanol
Description
Remarque sur l'analyse
Sibutramine, one of a few drugs approved for long-term treatment of obesity, is a chiral compound. The (R) enantiomer has the desired effect of reducing body weight and food intake, but the (S) enantiomer has the opposite effect: A perfect example of why chiral HPLC is important to pharmaceutical and clinical analysts. Astec® CHIROBIOTIC® V successfully separates sibutramine enantiomers, using a simple, MS-friendly mobile phase.
Informations légales
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC