HPLC Analysis of Ritalinic Acid and Ritalin (Methylphenidate) Enantiomers on Astec^® CHIROBIOTIC^® V2 with T2 Guard Column

column

CHIROBIOTIC T2 guard column, 2 cm x 4 mm I.D., 5 μm particles (16100AST (requires holder 21150AST))

column

CHIROBIOTIC V2, 15 cm x 4.6 mm I.D., 5 μm particles (15023AST)

mobile phase

[A] 20 mM ammonium acetate, pH 4.1; [B] methanol; (7:93, A:B)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 230 nm

injection

2 μL

sample

1 mg/mL in methanol