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  • A novel, low-volume method for organ culture of embryonic kidneys that allows development of cortico-medullary anatomical organization.

A novel, low-volume method for organ culture of embryonic kidneys that allows development of cortico-medullary anatomical organization.

PloS one (2010-05-19)
David D R Sebinger, Mathieu Unbekandt, Veronika V Ganeva, Andreas Ofenbauer, Carsten Werner, Jamie A Davies
RÉSUMÉ

Here, we present a novel method for culturing kidneys in low volumes of medium that offers more organotypic development compared to conventional methods. Organ culture is a powerful technique for studying renal development. It recapitulates many aspects of early development very well, but the established techniques have some disadvantages: in particular, they require relatively large volumes (1-3 mls) of culture medium, which can make high-throughput screens expensive, they require porous (filter) substrates which are difficult to modify chemically, and the organs produced do not achieve good cortico-medullary zonation. Here, we present a technique of growing kidney rudiments in very low volumes of medium-around 85 microliters-using silicone chambers. In this system, kidneys grow directly on glass, grow larger than in conventional culture and develop a clear anatomical cortico-medullary zonation with extended loops of Henle.

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Roche
Kit de détection in situ de la mort cellulaire, rouge TMR, sufficient for ≤50 tests
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1,2-Dioleoyl-sn-glycero-3-phosphocholine, lyophilized powder
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Glial Cell Line-derived Neurotrophic Factor human, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥98% (SDS-PAGE)